Lipid Modification Database
Tag Content
LipidDB ID
LipidDB-9913-01449
Entry Name
UniProt Accession
Theoretical PI
4.75
Molecular Weight
23727.88
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
Guanylyl cyclase-activating protein 2
Protein Synonyms/Alias
GCAP 2; Guanylate cyclase activator 1B; Retinal guanylyl cyclase activator protein p24;
Gene Name
GUCA1B
Gene Synonyms/Alias
GCAP-2; GCAP2;
Created Date
01-OCT-1996
 Lipid Modification Sites 
 Position   Sequence Form   Peptide   References   Modification Type 
2
Canonical
******MGQQFSWEE
[1][2]
N-Myristoylation
Organism
Bos taurus (Bovine)
NCBI Taxa ID
9913
Reference
[1] Theisgen S, Scheidt HA, Magalhães A, Bonagamba TJ, Huster D. A solid-state NMRstudy of the structure and dynamics of the myristoylated N-terminus of theguanylate cyclase-activating protein-2. Biochim Biophys Acta. 2010Feb;1798(2):266-74. doi: 10.1016/j.bbamem.2009.06.028. Epub 2009 Jul 17. Review.[PMID:19616509]
[2] Schröder T, Lilie H, Lange C. The myristoylation of guanylatecyclase-activating protein-2 causes an increase in thermodynamic stability in thepresence but not in the absence of Ca²⁺. Protein Sci. 2011 Jul;20(7):1155-65.doi: 10.1002/pro.643. Epub 2011 May 12.[PMID:21520322]
Functional Description
Stimulates guanylyl cyclase 1 (GC1) and GC2 when free calcium ions concentration is low, and GC1 and GC2 when free calcium ions concentration is elevated. This Ca(2+)-sensitive regulation of GC is a key event in recovery of the dark state of rod photoreceptors following light exposure.
Sequence Annotation
Domain: 19 54 EF-hand 1.
Domain: 56 91 EF-hand 2.
Domain: 92 127 EF-hand 3.
Domain: 145 180 EF-hand 4.
Protein Length
204 AA.
Protein Sequence
(Canonical)
MGQQFSWEEA EENGAVGAAD AAQLQEWYKK FLEECPSGTL FMHEFKRFFK VPDNEEATQY  60
VEAMFRAFDT NGDNTIDFLE YVAALNLVLR GTLEHKLKWT FKIYDKDRNG CIDRQELLDI  120
VESIYKLKKA CSVEVEAEQQ GKLLTPEEVV DRIFLLVDEN GDGQLSLNEF VEGARRDKWV  180
MKMLQMDLNP SSWISQQRRK SAMF                                         204
FASTA
(Canonical)
>LipidDB-9913-01449|P51177
MGQQFSWEEAEENGAVGAADAAQLQEWYKKFLEECPSGTLFMHEFKRFFKVPDNEEATQY
VEAMFRAFDTNGDNTIDFLEYVAALNLVLRGTLEHKLKWTFKIYDKDRNGCIDRQELLDI
VESIYKLKKACSVEVEAEQQGKLLTPEEVVDRIFLLVDENGDGQLSLNEFVEGARRDKWV
MKMLQMDLNPSSWISQQRRKSAMF
Gene Ontology
GO:0005886; C:plasma membrane; IEA:UniProtKB-KW
GO:0005509; F:calcium ion binding; IEA:InterPro
GO:0050896; P:response to stimulus; IEA:UniProtKB-KW
GO:0007601; P:visual perception; IEA:UniProtKB-KW
Interpro
InterPro; IPR011992; EF-hand-dom_pair
InterPro; IPR018247; EF_Hand_1_Ca_BS
InterPro; IPR002048; EF_hand_dom
InterPro; IPR015756; GCAP-2
InterPro; IPR028846; Recoverin
Pfam
Pfam; PF00036; EF-hand_1;
Pfam; PF13405; EF-hand_6;
SMART
SMART; SM00054; EFh;
PROSITE
PROSITE; PS00018; EF_HAND_1;
PROSITE; PS50222; EF_HAND_2;
PRINTS