Lipid Modification Database
Tag Content
LipidDB ID
LipidDB-9913-01060
Entry Name
UniProt Accession
Theoretical PI
5.48
Molecular Weight
39965.68
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
Guanine nucleotide-binding protein G(t) subunit alpha-1
Protein Synonyms/Alias
Transducin alpha-1 chain;
Gene Name
GNAT1
Gene Synonyms/Alias
Created Date
13-AUG-1987
 Lipid Modification Sites 
 Position   Sequence Form   Peptide   References   Modification Type 
2
Canonical
******MGAGASAEE
[1]
N-Myristoylation
Organism
Bos taurus (Bovine)
NCBI Taxa ID
9913
Reference
[1] Kokame K, Fukada Y, Yoshizawa T, Takao T, Shimonishi Y. Lipid modification at the N terminus of photoreceptor G-protein alpha-subunit. Nature. 1992 Oct22;359(6397):749-52.[PMID:1436039]
Functional Description
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase.
Sequence Annotation
Nucleotide-binding: 36 43 GTP.
Nucleotide-binding: 196 200 GTP.
Nucleotide-binding: 265 268 GTP.
Modified residue: 142 142 Phosphotyrosine.
Protein Length
350 AA.
Protein Sequence
(Canonical)
MGAGASAEEK HSRELEKKLK EDAEKDARTV KLLLLGAGES GKSTIVKQMK IIHQDGYSLE  60
ECLEFIAIIY GNTLQSILAI VRAMTTLNIQ YGDSARQDDA RKLMHMADTI EEGTMPKEMS  120
DIIQRLWKDS GIQACFDRAS EYQLNDSAGY YLSDLERLVT PGYVPTEQDV LRSRVKTTGI  180
IETQFSFKDL NFRMFDVGGQ RSERKKWIHC FEGVTCIIFI AALSAYDMVL VEDDEVNRMH  240
ESLHLFNSIC NHRYFATTSI VLFLNKKDVF SEKIKKAHLS ICFPDYNGPN TYEDAGNYIK  300
VQFLELNMRR DVKEIYSHMT CATDTQNVKF VFDAVTDIII KENLKDCGLF             350
FASTA
(Canonical)
>LipidDB-9913-01060|P04695
MGAGASAEEKHSRELEKKLKEDAEKDARTVKLLLLGAGESGKSTIVKQMKIIHQDGYSLE
ECLEFIAIIYGNTLQSILAIVRAMTTLNIQYGDSARQDDARKLMHMADTIEEGTMPKEMS
DIIQRLWKDSGIQACFDRASEYQLNDSAGYYLSDLERLVTPGYVPTEQDVLRSRVKTTGI
IETQFSFKDLNFRMFDVGGQRSERKKWIHCFEGVTCIIFIAALSAYDMVLVEDDEVNRMH
ESLHLFNSICNHRYFATTSIVLFLNKKDVFSEKIKKAHLSICFPDYNGPNTYEDAGNYIK
VQFLELNMRRDVKEIYSHMTCATDTQNVKFVFDAVTDIIIKENLKDCGLF
Gene Ontology
GO:0005834; C:heterotrimeric G-protein complex; IBA:RefGenome
GO:0032391; C:photoreceptor connecting cilium; IEA:Ensembl
GO:0097381; C:photoreceptor disc membrane; TAS:Reactome
GO:0042622; C:photoreceptor outer segment membrane; IDA:UniProtKB
GO:0000035; F:acyl binding; IDA:UniProtKB
GO:0031683; F:G-protein beta/gamma-subunit complex binding; IBA:RefGenome
GO:0001664; F:G-protein coupled receptor binding; IBA:RefGenome
GO:0019003; F:GDP binding; IDA:UniProtKB
GO:0005525; F:GTP binding; IDA:UniProtKB
GO:0003924; F:GTPase activity; IBA:RefGenome
GO:0019901; F:protein kinase binding; IDA:UniProtKB
GO:0004871; F:signal transducer activity; IBA:RefGenome
GO:0007188; P:adenylate cyclase-modulating G-protein coupled receptor signaling pathway; IBA:RefGenome
GO:0008283; P:cell proliferation; IEA:Ensembl
GO:0071257; P:cellular response to electrical stimulus; IEA:Ensembl
GO:0050908; P:detection of light stimulus involved in visual perception; IEA:Ensembl
GO:0042462; P:eye photoreceptor cell development; IEA:Ensembl
GO:0051344; P:negative regulation of cyclic-nucleotide phosphodiesterase activity; IDA:UniProtKB
GO:0007602; P:phototransduction; NAS:UniProtKB
GO:0007603; P:phototransduction, visible light; IEA:Ensembl
GO:0009416; P:response to light stimulus; IDA:UniProtKB
GO:0060041; P:retina development in camera-type eye; IEA:Ensembl
GO:0050917; P:sensory perception of umami taste; IBA:RefGenome
Interpro
InterPro; IPR001408; Gprotein_alpha_I
InterPro; IPR001019; Gprotein_alpha_su
InterPro; IPR011025; GproteinA_insert
InterPro; IPR027417; P-loop_NTPase
Pfam
Pfam; PF00503; G-alpha;
SMART
SMART; SM00275; G_alpha;
PROSITE
PRINTS
PRINTS; PR00318; GPROTEINA;
PRINTS; PR00441; GPROTEINAI;