LipidDB ID

LipidDB-9913-00841

Entry Name

UniProt Accession

P05131; P24256;

Theoretical PI

8.79

Molecular Weight

40593.71

Genbank Protein ID

AAA30707.1; AAA30424.1;

Genbank Nucleotide ID

J02647; M60482;

Protein Name

cAMP-dependent protein kinase catalytic subunit beta

Protein Synonyms/Alias

PKA C-beta; 2.7.11.11;

Gene Name

PRKACB

Gene Synonyms/Alias

Created Date

13-AUG-1987

Organism

Bos taurus (Bovine)

NCBI Taxa ID

9913

Reference

[1] Jedrzejewski PT, Girod A, Tholey A, König N, Thullner S, Kinzel V, Bossemeyer D. A conserved deamidation site at Asn 2 in the catalytic subunit of mammaliancAMP-dependent protein kinase detected by capillary LC-MS and tandem massspectrometry. Protein Sci. 1998 Feb;7(2):457-69.[PMID:9521123]

Functional Description

Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, and differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis (By similarity).

Sequence Annotation

Domain: 44 298 Protein kinase.
Domain: 299 351 AGC-kinase C-terminal.
Nucleotide-binding: 50 58 ATP.
Active site: 167 167 Proton acceptor.
Binding site: 73 73 ATP.
Modified residue: 3 3 Deamidated asparagine; partial.
Modified residue: 11 11 Phosphoserine.
Modified residue: 49 49 Phosphothreonine.
Modified residue: 69 69 Phosphotyrosine.
Modified residue: 140 140 Phosphoserine.
Modified residue: 196 196 Phosphothreonine.
Modified residue: 198 198 Phosphothreonine.
Modified residue: 202 202 Phosphothreonine.
Modified residue: 331 331 Phosphotyrosine.
Modified residue: 339 339 Phosphoserine.

Protein Length

351 AA.

Protein Sequence
(Canonical)

FASTA
(Canonical)

Gene Ontology

GO:0005813; C:centrosome; IEA:Ensembl
GO:0097546; C:ciliary base; IEA:Ensembl
GO:0005737; C:cytoplasm; IEA:UniProtKB-KW
GO:0070062; C:extracellular vesicular exosome; IEA:Ensembl
GO:0005634; C:nucleus; IEA:UniProtKB-KW
GO:0005886; C:plasma membrane; IEA:UniProtKB-KW
GO:0005524; F:ATP binding; IEA:UniProtKB-KW
GO:0004691; F:cAMP-dependent protein kinase activity; IEA:UniProtKB-EC
GO:0000287; F:magnesium ion binding; IEA:Ensembl
GO:1901621; P:negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterning; IEA:Ensembl
GO:0001843; P:neural tube closure; IEA:Ensembl
GO:0070613; P:regulation of protein processing; IEA:Ensembl

Interpro

InterPro; IPR000961; AGC-kinase_C
InterPro; IPR011009; Kinase-like_dom
InterPro; IPR000719; Prot_kinase_dom
InterPro; IPR017441; Protein_kinase_ATP_BS
InterPro; IPR002290; Ser/Thr_dual-sp_kinase
InterPro; IPR008271; Ser/Thr_kinase_AS

Pfam

Pfam; PF00069; Pkinase;

SMART

SMART; SM00133; S_TK_X;
SMART; SM00220; S_TKc;

PROSITE

PROSITE; PS51285; AGC_KINASE_CTER;
PROSITE; PS00107; PROTEIN_KINASE_ATP;
PROSITE; PS50011; PROTEIN_KINASE_DOM;
PROSITE; PS00108; PROTEIN_KINASE_ST;

PRINTS