LipidDB ID

LipidDB-9913-00014

Entry Name

UniProt Accession

Q28175; Q05661;

Theoretical PI

6.0

Molecular Weight

60944.4

Genbank Protein ID

AAC37306.1; CAA46988.1;

Genbank Nucleotide ID

L11356; X66277;

Protein Name

Retinoid isomerohydrolase

Protein Synonyms/Alias

3.1.1.64; All-trans-retinyl-palmitate hydrolase; Retinal pigment epithelium-specific 65 kDa protein; Retinol isomerase;

Gene Name

RPE65

Gene Synonyms/Alias

Created Date

10-OCT-2003

Organism

Bos taurus (Bovine)

NCBI Taxa ID

9913

Reference

[1] Takahashi Y, Moiseyev G, Ablonczy Z, Chen Y, Crouch RK, Ma JX. Identification of a novel palmitylation site essential for membrane association andisomerohydrolase activity of RPE65. J Biol Chem. 2009 Jan 30;284(5):3211-8. doi: 10.1074/jbc.M807248200. Epub 2008 Dec 1.[PMID:19049981]

Functional Description

Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis- retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association.

Sequence Annotation

Metal binding site: 180 180 Iron; catalytic.
Metal binding site: 241 241 Iron; catalytic.
Metal binding site: 313 313 Iron; catalytic.
Metal binding site: 527 527 Iron; catalytic.
Modified residue: 2 2 N-acetylserine.
Modified residue: 101 101 Phosphothreonine.
Modified residue: 105 105 Phosphothreonine.
Modified residue: 113 113 N6-acetyllysine.
Modified residue: 117 117 Phosphoserine.

Protein Length

533 AA.

Protein Sequence
(Canonical)

FASTA
(Canonical)

Gene Ontology

GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-KW
GO:0005886; C:plasma membrane; IEA:UniProtKB-KW
GO:0052885; F:all-trans-retinyl-ester hydrolase, 11-cis retinol forming activity; IEA:UniProtKB-EC
GO:0052884; F:all-trans-retinyl-palmitate hydrolase, 11-cis retinol forming activity; IEA:UniProtKB-EC
GO:0046872; F:metal ion binding; IEA:UniProtKB-KW
GO:0004744; F:retinal isomerase activity; IEA:Ensembl
GO:0071257; P:cellular response to electrical stimulus; IEA:Ensembl
GO:0050908; P:detection of light stimulus involved in visual perception; IEA:Ensembl
GO:0008286; P:insulin receptor signaling pathway; IEA:Ensembl
GO:0007468; P:regulation of rhodopsin gene expression; IEA:Ensembl
GO:0001895; P:retina homeostasis; IEA:Ensembl
GO:0060042; P:retina morphogenesis in camera-type eye; IEA:Ensembl
GO:0042574; P:retinal metabolic process; IEA:Ensembl

Interpro

InterPro; IPR004294; Carotenoid_Oase

Pfam

Pfam; PF03055; RPE65;

SMART

PROSITE

PRINTS