Lipid Modification Database
Tag Content
LipidDB ID
LipidDB-9606-01471
Entry Name
UniProt Accession
Theoretical PI
6.31
Molecular Weight
20696.77
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
ADP-ribosylation factor 1
Protein Synonyms/Alias
Gene Name
ARF1
Gene Synonyms/Alias
Created Date
16-AUG-2004
 Lipid Modification Sites 
 Position   Sequence Form   Peptide   References   Modification Type 
2
Canonical
******MGNIFANLF
[1][2]
N-Myristoylation
Organism
Homo sapiens (Human)
NCBI Taxa ID
9606
Reference
[1] Burnaevskiy N, Fox TG, Plymire DA, Ertelt JM, Weigele BA, Selyunin AS, Way SS,Patrie SM, Alto NM. Proteolytic elimination of N-myristoyl modifications by theShigella virulence factor IpaJ. Nature. 2013 Apr 4;496(7443):106-9. doi:10.1038/nature12004. Epub 2013 Mar 27.[PMID:23535599]
[2] Suzuki T, Moriya K, Nagatoshi K, Ota Y, Ezure T, Ando E, Tsunasawa S, UtsumiT. Strategy for comprehensive identification of human N-myristoylated proteinsusing an insect cell-free protein synthesis system. Proteomics. 2010May;10(9):1780-93. doi: 10.1002/pmic.200900783.[PMID:20213681]
Functional Description
GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression (LTD).
Sequence Annotation
Nucleotide-binding: 24 31 GTP.
Nucleotide-binding: 126 129 GTP.
Modified residue: 2 2 N-acetylglycine; alternate.
Protein Length
181 AA.
Protein Sequence
(Canonical)
MGNIFANLFK GLFGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN  60
ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV NEAREELMRM LAEDELRDAV  120
LLVFANKQDL PNAMNAAEIT DKLGLHSLRH RNWYIQATCA TSGDGLYEGL DWLSNQLRNQ  180
K                                                                  181
FASTA
(Canonical)
>LipidDB-9606-01471|P84077
MGNIFANLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKN
ISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAV
LLVFANKQDLPNAMNAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLSNQLRNQ
K
Gene Ontology
GO:0005829; C:cytosol; TAS:Reactome
GO:0070062; C:extracellular vesicular exosome; IDA:UniProtKB
GO:0005925; C:focal adhesion; IDA:UniProtKB
GO:0000139; C:Golgi membrane; TAS:Reactome
GO:0043005; C:neuron projection; IEA:UniProtKB-KW
GO:0005886; C:plasma membrane; TAS:ProtInc
GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-KW
GO:0030017; C:sarcomere; IEA:Ensembl
GO:0005525; F:GTP binding; IEA:UniProtKB-KW
GO:0003924; F:GTPase activity; TAS:ProtInc
GO:0044822; F:poly(A) RNA binding; IDA:UniProtKB
GO:0005057; F:receptor signaling protein activity; TAS:ProtInc
GO:0019886; P:antigen processing and presentation of exogenous peptide antigen via MHC class II; TAS:Reactome
GO:0006878; P:cellular copper ion homeostasis; IMP:UniProtKB
GO:0048205; P:COPI coating of Golgi vesicle; TAS:Reactome
GO:0097061; P:dendritic spine organization; ISS:UniProtKB
GO:0006184; P:GTP catabolic process; TAS:GOC
GO:0060292; P:long term synaptic depression; ISS:UniProtKB
GO:0061024; P:membrane organization; TAS:Reactome
GO:0006661; P:phosphatidylinositol biosynthetic process; TAS:Reactome
GO:0006644; P:phospholipid metabolic process; TAS:Reactome
GO:0006892; P:post-Golgi vesicle-mediated transport; TAS:Reactome
GO:0015031; P:protein transport; IEA:UniProtKB-KW
GO:0034315; P:regulation of Arp2/3 complex-mediated actin nucleation; ISS:UniProtKB
GO:0050690; P:regulation of defense response to virus by virus; TAS:Reactome
GO:0002090; P:regulation of receptor internalization; ISS:UniProtKB
GO:0006890; P:retrograde vesicle-mediated transport, Golgi to ER; TAS:Reactome
GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro
GO:0044281; P:small molecule metabolic process; TAS:Reactome
GO:0016032; P:viral process; TAS:Reactome
Interpro
InterPro; IPR027417; P-loop_NTPase
InterPro; IPR005225; Small_GTP-bd_dom
InterPro; IPR024156; Small_GTPase_ARF
InterPro; IPR006689; Small_GTPase_ARF/SAR
Pfam
Pfam; PF00025; Arf;
SMART
SMART; SM00177; ARF;
PROSITE
PROSITE; PS51417; ARF;
PRINTS
PRINTS; PR00328; SAR1GTPBP;