LipidDB ID

LipidDB-9606-01304

Entry Name

UniProt Accession

Q9BZQ4; O75067; Q5T1Q3; Q8WU99; Q96QW1;

Theoretical PI

6.59

Molecular Weight

34438.63

Genbank Protein ID

AAG60615.1; BAA32324.1; CAH70979.1; CAH70979.1; CAH70982.1; CAH70982.1; CAH70982.1; CAI15467.1; CAI15467.1; CAI15468.1; CAI15468.1; CAI15468.1; CAI16624.1; CAI16624.1; CAI16624.1; AAH20998.1;

Genbank Nucleotide ID

AF288395; AB007948; AL354953; AL356981; AL354953; AL356981; AL449223; AL356981; AL354953; AL356981; AL354953; AL449223; AL449223; AL354953; AL356981; BC020998;

Protein Name

Nicotinamide mononucleotide adenylyltransferase 2

Protein Synonyms/Alias

NMN adenylyltransferase 2; 2.7.7.1; Nicotinate-nucleotide adenylyltransferase 2; NaMN adenylyltransferase 2; 2.7.7.18;

Gene Name

NMNAT2

Gene Synonyms/Alias

C1orf15; KIAA0479;

Created Date

09-MAY-2003

Organism

Homo sapiens (Human)

NCBI Taxa ID

9606

Reference

[1] Lau C, Dölle C, Gossmann TI, Agledal L, Niere M, Ziegler M. Isoform-specifictargeting and interaction domains in human nicotinamide mononucleotideadenylyltransferases. J Biol Chem. 2010 Jun 11;285(24):18868-76. doi:10.1074/jbc.M110.107631. Epub 2010 Apr 13.[PMID:20388704]
[2] Mayer PR, Huang N, Dewey CM, Dries DR, Zhang H, Yu G. Expression,localization, and biochemical characterization of nicotinamide mononucleotideadenylyltransferase 2. J Biol Chem. 2010 Dec 17;285(51):40387-96. doi:10.1074/jbc.M110.178913. Epub 2010 Oct 13.[PMID:20943658]

Functional Description

Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate but with a lower efficiency. Cannot use triazofurin monophosphate (TrMP) as substrate. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity prefers NAD(+), NADH and NAAD as substrates and degrades nicotinic acid adenine dinucleotide phosphate (NHD) less effectively. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NAADP(+).

Sequence Annotation

Nucleotide-binding: 15 24 ATP.
Nucleotide-binding: 269 274 ATP.
Binding site: 16 16 Substrate.
Binding site: 55 55 Substrate.
Binding site: 202 202 Substrate.

Protein Length

307 AA.

Protein Sequence
(Canonical)

FASTA
(Canonical)

Gene Ontology

GO:0005794; C:Golgi apparatus; IDA:UniProtKB
GO:0000139; C:Golgi membrane; TAS:Reactome
GO:0005770; C:late endosome; IEA:Ensembl
GO:0045202; C:synapse; IEA:Ensembl
GO:0005802; C:trans-Golgi network; IEA:Ensembl
GO:0005524; F:ATP binding; IEA:UniProtKB-KW
GO:0000309; F:nicotinamide-nucleotide adenylyltransferase activity; TAS:Reactome
GO:0004515; F:nicotinate-nucleotide adenylyltransferase activity; IDA:UniProtKB
GO:0009435; P:NAD biosynthetic process; IC:UniProtKB
GO:0019674; P:NAD metabolic process; TAS:Reactome
GO:0044281; P:small molecule metabolic process; TAS:Reactome
GO:0006766; P:vitamin metabolic process; TAS:Reactome
GO:0006767; P:water-soluble vitamin metabolic process; TAS:Reactome

Interpro

InterPro; IPR004821; Cyt_trans-like
InterPro; IPR005248; NAMN_adtrnsfrase
InterPro; IPR014729; Rossmann-like_a/b/a_fold

Pfam

Pfam; PF01467; CTP_transf_2;

SMART

PROSITE

PRINTS