LipidDB ID

LipidDB-9606-00907

Entry Name

UniProt Accession

P08631; A8K1I1; B4DQB6; E1P5M2; Q29RX1; Q2VPE2; Q504R5; Q5T7K1; Q5T7K2; Q96CC0; Q9H5Y5; Q9NUA4; Q9UMJ5;

Theoretical PI

6.27

Molecular Weight

59599.84

Genbank Protein ID

AAA52643.1; AAA52644.1; BAB15482.1; BAF82585.1; BAG60878.1; CAI19694.1; CAI19694.1; CAI19695.1; CAI19695.1; CAI22966.1; CAI22966.1; CAI22967.1; CAI22967.1; EAW76392.1; EAW76393.1; AAH14435.2; AAH94847.2; AAI08931.2; AAI08932.2; AAI13855.2; AAI14464.2; CAA41565.2; CAA41565.2; CAA41565.2;

Genbank Nucleotide ID

M16591; M16592; AK026432; AK289896; AK298726; AL353092; AL049539; AL353092; AL049539; AL049539; AL353092; AL049539; AL353092; CH471077; CH471077; BC014435; BC094847; BC108930; BC108931; BC113854; BC114463; X58741; X58742; X58743;

Protein Name

Tyrosine-protein kinase HCK

Protein Synonyms/Alias

2.7.10.2; Hematopoietic cell kinase; Hemopoietic cell kinase; p59-HCK/p60-HCK; p59Hck; p61Hck;

Gene Name

HCK

Gene Synonyms/Alias

Created Date

01-AUG-1988

Organism

Homo sapiens (Human)

NCBI Taxa ID

9606

Reference

[1] Robbins SM, Quintrell NA, Bishop JM. Myristoylation and differentialpalmitoylation of the HCK protein-tyrosine kinases govern their attachment tomembranes and association with caveolae. Mol Cell Biol. 1995 Jul;15(7):3507-15.[PMID:7791757]

Functional Description

Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS.

Sequence Annotation

Domain: 78 138 SH3.
Domain: 144 241 SH2.
Domain: 262 515 Protein kinase.
Nucleotide-binding: 268 276 ATP.
Active site: 381 381 Proton acceptor.
Binding site: 290 290 ATP.
Modified residue: 51 51 Phosphotyrosine; by autocatalysis.
Modified residue: 202 202 Phosphothreonine.
Modified residue: 209 209 Phosphotyrosine.
Modified residue: 411 411 Phosphotyrosine; by autocatalysis.
Modified residue: 462 462 Phosphoserine.
Modified residue: 522 522 Phosphotyrosine.

Protein Length

526 AA.

Protein Sequence
(Canonical)

FASTA
(Canonical)

Gene Ontology

GO:0005901; C:caveola; IDA:UniProtKB
GO:0042995; C:cell projection; IEA:UniProtKB-KW
GO:0031410; C:cytoplasmic vesicle; IEA:UniProtKB-KW
GO:0005856; C:cytoskeleton; IEA:UniProtKB-KW
GO:0005829; C:cytosol; TAS:Reactome
GO:0031234; C:extrinsic component of cytoplasmic side of plasma membrane; IMP:UniProtKB
GO:0005925; C:focal adhesion; IMP:UniProtKB
GO:0005794; C:Golgi apparatus; IEA:UniProtKB-KW
GO:0005764; C:lysosome; IDA:UniProtKB
GO:0005634; C:nucleus; IEA:UniProtKB-KW
GO:0005524; F:ATP binding; IEA:UniProtKB-KW
GO:0004715; F:non-membrane spanning protein tyrosine kinase activity; IEA:UniProtKB-EC
GO:0004713; F:protein tyrosine kinase activity; IMP:UniProtKB
GO:0007155; P:cell adhesion; TAS:UniProtKB
GO:0019221; P:cytokine-mediated signaling pathway; TAS:UniProtKB
GO:0038096; P:Fc-gamma receptor signaling pathway involved in phagocytosis; TAS:Reactome
GO:0006954; P:inflammatory response; IEA:UniProtKB-KW
GO:0045087; P:innate immune response; TAS:Reactome
GO:0002758; P:innate immune response-activating signal transduction; TAS:UniProtKB
GO:0007229; P:integrin-mediated signaling pathway; TAS:UniProtKB
GO:0060333; P:interferon-gamma-mediated signaling pathway; TAS:UniProtKB
GO:0043299; P:leukocyte degranulation; TAS:UniProtKB
GO:0002522; P:leukocyte migration involved in immune response; TAS:UniProtKB
GO:0031663; P:lipopolysaccharide-mediated signaling pathway; TAS:UniProtKB
GO:0007498; P:mesoderm development; TAS:ProtInc
GO:0043066; P:negative regulation of apoptotic process; IMP:UniProtKB
GO:0018108; P:peptidyl-tyrosine phosphorylation; IMP:UniProtKB
GO:2000251; P:positive regulation of actin cytoskeleton reorganization; IDA:UniProtKB
GO:0030838; P:positive regulation of actin filament polymerization; TAS:UniProtKB
GO:0008284; P:positive regulation of cell proliferation; IMP:UniProtKB
GO:0046777; P:protein autophosphorylation; IMP:UniProtKB
GO:0006468; P:protein phosphorylation; TAS:ProtInc
GO:0008360; P:regulation of cell shape; IMP:UniProtKB
GO:0050690; P:regulation of defense response to virus by virus; TAS:Reactome
GO:0050727; P:regulation of inflammatory response; TAS:UniProtKB
GO:0050764; P:regulation of phagocytosis; IMP:UniProtKB
GO:0071801; P:regulation of podosome assembly; IDA:UniProtKB
GO:0051090; P:regulation of sequence-specific DNA binding transcription factor activity; IMP:UniProtKB
GO:0045728; P:respiratory burst after phagocytosis; TAS:UniProtKB
GO:0016032; P:viral process; TAS:Reactome

Interpro

InterPro; IPR011009; Kinase-like_dom
InterPro; IPR000719; Prot_kinase_dom
InterPro; IPR017441; Protein_kinase_ATP_BS
InterPro; IPR001245; Ser-Thr/Tyr_kinase_cat_dom
InterPro; IPR000980; SH2
InterPro; IPR001452; SH3_domain
InterPro; IPR008266; Tyr_kinase_AS
InterPro; IPR020635; Tyr_kinase_cat_dom

Pfam

Pfam; PF07714; Pkinase_Tyr;
Pfam; PF00017; SH2;
Pfam; PF00018; SH3_1;

SMART

SMART; SM00252; SH2;
SMART; SM00326; SH3;
SMART; SM00219; TyrKc;

PROSITE

PROSITE; PS00107; PROTEIN_KINASE_ATP;
PROSITE; PS50011; PROTEIN_KINASE_DOM;
PROSITE; PS00109; PROTEIN_KINASE_TYR;
PROSITE; PS50001; SH2;
PROSITE; PS50002; SH3;

PRINTS

PRINTS; PR00401; SH2DOMAIN;
PRINTS; PR00452; SH3DOMAIN;
PRINTS; PR00109; TYRKINASE;