LipidDB ID

LipidDB-9606-00744

Entry Name

UniProt Accession

P09936; Q4W5K6; Q71UM0;

Theoretical PI

5.33

Molecular Weight

24824.34

Genbank Protein ID

AAY40923.1; EAW92983.1; AAH00332.1; AAH05117.1; AAH06305.1; CAA35249.1; CAA28443.1; AAD09172.1;

Genbank Nucleotide ID

AC095043; CH471069; BC000332; BC005117; BC006305; X17377; X04741; AH007277;

Protein Name

Ubiquitin carboxyl-terminal hydrolase isozyme L1

Protein Synonyms/Alias

UCH-L1; 3.4.19.12; 6.-.-.-; Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1;

Gene Name

UCHL1

Gene Synonyms/Alias

Created Date

01-JUL-1989

Organism

Homo sapiens (Human)

NCBI Taxa ID

9606

Reference

[1] Liu Z, Meray RK, Grammatopoulos TN, Fredenburg RA, Cookson MR, Liu Y, Logan T,Lansbury PT Jr. Membrane-associated farnesylated UCH-L1 promotes alpha-synuclein neurotoxicity and is a therapeutic target for Parkinson's disease. Proc Natl AcadSci U S A. 2009 Mar 24;106(12):4635-40. doi: 10.1073/pnas.0806474106. Epub 2009Mar 4.[PMID:19261853]

Functional Description

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.

Sequence Annotation

Region: 5 10 Interaction with ubiquitin.
Region: 211 216 Interaction with ubiquitin.
Active site: 90 90 Nucleophile.
Active site: 161 161 Proton donor.
Functional site: 1 1 Susceptible to oxidation.
Functional site: 6 6 Susceptible to oxidation.
Functional site: 12 12 Susceptible to oxidation.
Functional site: 124 124 Susceptible to oxidation.
Functional site: 176 176 Important for enzyme activity.
Functional site: 179 179 Susceptible to oxidation.
Functional site: 220 220 Susceptible to oxidation.

Protein Length

223 AA.

Protein Sequence
(Canonical)

FASTA
(Canonical)

Gene Ontology

GO:0030424; C:axon; IEA:Ensembl
GO:0005737; C:cytoplasm; ISS:UniProtKB
GO:0005829; C:cytosol; IEA:Ensembl
GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-KW
GO:0070062; C:extracellular vesicular exosome; IDA:UniProt
GO:0016020; C:membrane; IEA:UniProtKB-KW
GO:0043025; C:neuronal cell body; IEA:Ensembl
GO:0005634; C:nucleus; IDA:HPA
GO:0031694; F:alpha-2A adrenergic receptor binding; IPI:BHF-UCL
GO:0004197; F:cysteine-type endopeptidase activity; IDA:UniProtKB
GO:0016874; F:ligase activity; IEA:UniProtKB-KW
GO:0008242; F:omega peptidase activity; IDA:UniProtKB
GO:0043130; F:ubiquitin binding; IDA:UniProtKB
GO:0004221; F:ubiquitin thiolesterase activity; TAS:UniProtKB
GO:0004843; F:ubiquitin-specific protease activity; IEA:InterPro
GO:0007628; P:adult walking behavior; IEA:Ensembl
GO:0007412; P:axon target recognition; IEA:Ensembl
GO:0019896; P:axon transport of mitochondrion; IEA:Ensembl
GO:0008219; P:cell death; IEA:UniProtKB-KW
GO:0008283; P:cell proliferation; IEA:Ensembl
GO:0042755; P:eating behavior; IEA:Ensembl
GO:0048747; P:muscle fiber development; IEA:Ensembl
GO:0043407; P:negative regulation of MAP kinase activity; IDA:BHF-UCL
GO:0050905; P:neuromuscular process; IEA:Ensembl
GO:0016579; P:protein deubiquitination; IDA:UniProtKB
GO:0002931; P:response to ischemia; IEA:Ensembl
GO:0019233; P:sensory perception of pain; IEA:Ensembl
GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:Ensembl

Interpro

InterPro; IPR001578; Peptidase_C12_UCH

Pfam

Pfam; PF01088; Peptidase_C12;

SMART

PROSITE

PROSITE; PS00140; UCH_1;

PRINTS

PRINTS; PR00707; UBCTHYDRLASE;