LipidDB ID

LipidDB-9606-00630

Entry Name

UniProt Accession

P63000; O95501; P15154; Q3Y4D3; Q5JAA8; Q9BTB4;

Theoretical PI

8.77

Molecular Weight

21450.11

Genbank Protein ID

AAA36537.1; AAA36544.1; CAA10732.1; CAB53579.5; CAA10733.6; AAD30547.1; AAQ16632.1; AAM21111.1; AAP35785.1; AAZ80485.1; AAS07511.1; AAS07512.1; AAH04247.1; AAH50687.1; AAI07749.1;

Genbank Nucleotide ID

M29870; M31467; AJ132694; AJ132695; AJ132695; AF136373; AY279384; AF498964; BT007121; DQ165078; AC009412; AC009412; BC004247; BC050687; BC107748;

Protein Name

Ras-related C3 botulinum toxin substrate 1

Protein Synonyms/Alias

Cell migration-inducing gene 5 protein; Ras-like protein TC25; p21-Rac1;

Gene Name

RAC1

Gene Synonyms/Alias

TC25; MIG5;

Created Date

31-AUG-2004

Organism

Homo sapiens (Human)

NCBI Taxa ID

9606

Reference

[1] Navarro-Lérida I, Sánchez-Perales S, Calvo M, Rentero C, Zheng Y, Enrich C,Del Pozo MA. A palmitoylation switch mechanism regulates Rac1 function andmembrane organization. EMBO J. 2012 Feb 1;31(3):534-51. doi:10.1038/emboj.2011.446. Epub 2011 Dec 9.[PMID:22157745]
[2] Kinsella BT, Erdman RA, Maltese WA. Carboxyl-terminal isoprenylation ofras-related GTP-binding proteins encoded by rac1, rac2, and ralA. J Biol Chem.1991 May 25;266(15):9786-94.[PMID:1903399]

Functional Description

Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion. In podocytes, promotes nuclear shuttling of NR3C2; this modulation is required for a proper kidney functioning. Required for atypical chemokine receptor ACKR2-induced LIMK1-PAK1-dependent phosphorylation of cofilin (CFL1) and for up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3.

Sequence Annotation

Nucleotide-binding: 10 17 GTP.
Nucleotide-binding: 57 61 GTP.
Nucleotide-binding: 115 118 GTP.
Motif: 32 40 Effector region.
Modified residue: 32 32 O-AMP-tyrosine; by Haemophilus IbpA.
Modified residue: 35 35 O-AMP-threonine; by Vibrio VopS.
Modified residue: 39 39 ADP-ribosylasparagine; by botulinumtoxin.
Modified residue: 189 189 Cysteine methyl ester.

Protein Length

192 AA.

Protein Sequence
(Canonical)

FASTA
(Canonical)

Gene Ontology

GO:0005737; C:cytoplasm; IDA:UniProtKB
GO:0016023; C:cytoplasmic membrane-bounded vesicle; IEA:Ensembl
GO:0036464; C:cytoplasmic ribonucleoprotein granule; IDA:ParkinsonsUK-UCL
GO:0005829; C:cytosol; ISS:UniProtKB
GO:0070062; C:extracellular vesicular exosome; IDA:UniProtKB
GO:0019897; C:extrinsic component of plasma membrane; IEA:Ensembl
GO:0005925; C:focal adhesion; IDA:UniProtKB
GO:0000139; C:Golgi membrane; IEA:Ensembl
GO:0030027; C:lamellipodium; IDA:UniProtKB
GO:0016020; C:membrane; ISS:UniProtKB
GO:0001891; C:phagocytic cup; IEA:Ensembl
GO:0005886; C:plasma membrane; TAS:Reactome
GO:0032587; C:ruffle membrane; IEA:Ensembl
GO:0005802; C:trans-Golgi network; IDA:FlyBase
GO:0019899; F:enzyme binding; IPI:BHF-UCL
GO:0005525; F:GTP binding; IDA:UniProtKB
GO:0003924; F:GTPase activity; TAS:UniProtKB
GO:0051022; F:Rho GDP-dissociation inhibitor binding; ISS:UniProtKB
GO:0031996; F:thioesterase binding; IPI:UniProtKB
GO:0030036; P:actin cytoskeleton organization; IGI:MGI
GO:0030041; P:actin filament polymerization; TAS:UniProtKB
GO:0048532; P:anatomical structure arrangement; IEA:Ensembl
GO:0009653; P:anatomical structure morphogenesis; TAS:ProtInc
GO:0097190; P:apoptotic signaling pathway; TAS:Reactome
GO:0002093; P:auditory receptor cell morphogenesis; IEA:Ensembl
GO:0007411; P:axon guidance; TAS:Reactome
GO:0007596; P:blood coagulation; TAS:Reactome
GO:0045453; P:bone resorption; IEA:Ensembl
GO:0007155; P:cell adhesion; TAS:ProtInc
GO:0048870; P:cell motility; IDA:UniProtKB
GO:0008283; P:cell proliferation; IEA:Ensembl
GO:0045216; P:cell-cell junction organization; IEA:Ensembl
GO:0007160; P:cell-matrix adhesion; NAS:BHF-UCL
GO:0006928; P:cellular component movement; TAS:ProtInc
GO:0021799; P:cerebral cortex radially oriented cell migration; IEA:Ensembl
GO:0090103; P:cochlea morphogenesis; IEA:Ensembl
GO:0048813; P:dendrite morphogenesis; IEA:Ensembl
GO:0071542; P:dopaminergic neuron differentiation; IEA:Ensembl
GO:0021831; P:embryonic olfactory bulb interneuron precursor migration; IEA:Ensembl
GO:0043652; P:engulfment of apoptotic cell; IEA:Ensembl
GO:0003382; P:epithelial cell morphogenesis; IEA:Ensembl
GO:0038095; P:Fc-epsilon receptor signaling pathway; TAS:Reactome
GO:0038096; P:Fc-gamma receptor signaling pathway involved in phagocytosis; TAS:Reactome
GO:0007186; P:G-protein coupled receptor signaling pathway; IEA:Ensembl
GO:0006972; P:hyperosmotic response; IEA:Ensembl
GO:0006954; P:inflammatory response; TAS:ProtInc
GO:0045087; P:innate immune response; TAS:Reactome
GO:0035556; P:intracellular signal transduction; TAS:ProtInc
GO:0030032; P:lamellipodium assembly; IMP:UniProtKB
GO:0051668; P:localization within membrane; IMP:BHF-UCL
GO:0002551; P:mast cell chemotaxis; IEA:Ensembl
GO:0032707; P:negative regulation of interleukin-23 production; IDA:BHF-UCL
GO:0048261; P:negative regulation of receptor-mediated endocytosis; TAS:UniProtKB
GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome
GO:0030168; P:platelet activation; TAS:Reactome
GO:0030838; P:positive regulation of actin filament polymerization; IEA:Ensembl
GO:0043065; P:positive regulation of apoptotic process; TAS:Reactome
GO:0045740; P:positive regulation of DNA replication; IEA:Ensembl
GO:0051894; P:positive regulation of focal adhesion assembly; IDA:UniProtKB
GO:0010592; P:positive regulation of lamellipodium assembly; IMP:UniProtKB
GO:0090023; P:positive regulation of neutrophil chemotaxis; IMP:UniProtKB
GO:0043552; P:positive regulation of phosphatidylinositol 3-kinase activity; IEA:Ensembl
GO:0001934; P:positive regulation of protein phosphorylation; IMP:UniProtKB
GO:0035025; P:positive regulation of Rho protein signal transduction; TAS:UniProtKB
GO:0051496; P:positive regulation of stress fiber assembly; IDA:UniProtKB
GO:1900026; P:positive regulation of substrate adhesion-dependent cell spreading; IDA:UniProtKB
GO:0072659; P:protein localization to plasma membrane; IEA:Ensembl
GO:0030334; P:regulation of cell migration; IMP:UniProtKB
GO:0050690; P:regulation of defense response to virus by virus; TAS:Reactome
GO:0010310; P:regulation of hydrogen peroxide metabolic process; TAS:BHF-UCL
GO:0060263; P:regulation of respiratory burst; IDA:BHF-UCL
GO:0009611; P:response to wounding; TAS:ProtInc
GO:0097178; P:ruffle assembly; IEA:Ensembl
GO:0031529; P:ruffle organization; TAS:UniProtKB
GO:0071526; P:semaphorin-plexin signaling pathway; ISS:UniProtKB
GO:0007264; P:small GTPase mediated signal transduction; IEA:Ensembl
GO:0034446; P:substrate adhesion-dependent cell spreading; IMP:UniProtKB
GO:0031295; P:T cell costimulation; TAS:Reactome
GO:0016032; P:viral process; TAS:Reactome
GO:0060071; P:Wnt signaling pathway, planar cell polarity pathway; IEA:Ensembl

Interpro

InterPro; IPR027417; P-loop_NTPase
InterPro; IPR005225; Small_GTP-bd_dom
InterPro; IPR001806; Small_GTPase
InterPro; IPR003578; Small_GTPase_Rho

Pfam

Pfam; PF00071; Ras;

SMART

SMART; SM00174; RHO;

PROSITE

PROSITE; PS51420; RHO;

PRINTS

PRINTS; PR00449; RASTRNSFRMNG;