LipidDB ID

LipidDB-9606-00553

Entry Name

UniProt Accession

P31689; Q5T7Q0; Q86TL9;

Theoretical PI

6.65

Molecular Weight

44868.18

Genbank Protein ID

BAA02656.1; AAC37517.1; AAO31694.1; AAP35956.1; BAF82312.1; EAW58525.1; EAW58526.1; AAH08182.1;

Genbank Nucleotide ID

D13388; L08069; AY186741; BT007292; AK289623; CH471071; CH471071; BC008182;

Protein Name

DnaJ homolog subfamily A member 1

Protein Synonyms/Alias

DnaJ protein homolog 2; HSDJ; Heat shock 40 kDa protein 4; Heat shock protein J2; HSJ-2; Human DnaJ protein 2; hDj-2;

Gene Name

DNAJA1

Gene Synonyms/Alias

DNAJ2; HDJ2; HSJ2; HSPF4;

Created Date

01-JUL-1993

Organism

Homo sapiens (Human)

NCBI Taxa ID

9606

Reference

[1] Kanazawa M, Terada K, Kato S, Mori M. HSDJ, a human homolog of DnaJ, isfarnesylated and is involved in protein import into mitochondria. J Biochem. 1997May;121(5):890-5.[PMID:9192730]

Functional Description

Co-chaperone for HSPA8/Hsc70 (PubMed:10816573). Stimulates ATP hydrolysis, but not the folding of unfolded proteins mediated by HSPA1A (in vitro) (PubMed:24318877). Plays a role in protein transport into mitochondria via its role as co- chaperone. Functions as co-chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis (PubMed:14752510). Promotes apoptosis in response to cellular stress mediated by exposure to anisomycin or UV (PubMed:24512202).

Sequence Annotation

Domain: 6 68 J.
Metal binding site: 134 134 Zinc 1.
Metal binding site: 137 137 Zinc 1.
Metal binding site: 150 150 Zinc 2.
Metal binding site: 153 153 Zinc 2.
Metal binding site: 177 177 Zinc 2.
Metal binding site: 180 180 Zinc 2.
Metal binding site: 193 193 Zinc 1.
Metal binding site: 196 196 Zinc 1.
Modified residue: 66 66 N6-acetyllysine.
Modified residue: 83 83 Phosphoserine.
Modified residue: 335 335 Phosphoserine.
Modified residue: 381 381 Phosphotyrosine.
Modified residue: 394 394 Cysteine methyl ester.

Protein Length

397 AA.

Protein Sequence
(Canonical)

FASTA
(Canonical)

Gene Ontology

GO:0098554; C:cytoplasmic side of endoplasmic reticulum membrane; TAS:ParkinsonsUK-UCL
GO:0005829; C:cytosol; IDA:UniProt
GO:0070062; C:extracellular vesicular exosome; IDA:UniProt
GO:0016020; C:membrane; IDA:UniProtKB
GO:0005739; C:mitochondrion; IEA:UniProtKB-KW
GO:0005634; C:nucleus; IEA:UniProtKB-KW
GO:0005524; F:ATP binding; IEA:InterPro
GO:0051087; F:chaperone binding; IDA:UniProtKB
GO:0001664; F:G-protein coupled receptor binding; IPI:ParkinsonsUK-UCL
GO:0030544; F:Hsp70 protein binding; IDA:UniProtKB
GO:0050750; F:low-density lipoprotein particle receptor binding; IDA:MGI
GO:0046872; F:metal ion binding; IEA:UniProtKB-KW
GO:0031625; F:ubiquitin protein ligase binding; IPI:ParkinsonsUK-UCL
GO:0030521; P:androgen receptor signaling pathway; IEA:Ensembl
GO:0042769; P:DNA damage response, detection of DNA damage; IEA:Ensembl
GO:0043066; P:negative regulation of apoptotic process; IMP:UniProtKB
GO:0043508; P:negative regulation of JUN kinase activity; IMP:UniProtKB
GO:0031397; P:negative regulation of protein ubiquitination; IDA:ParkinsonsUK-UCL
GO:0043065; P:positive regulation of apoptotic process; IMP:UniProtKB
GO:0006457; P:protein folding; TAS:ProtInc
GO:0070585; P:protein localization to mitochondrion; IMP:UniProtKB
GO:0051223; P:regulation of protein transport; IDA:UniProtKB
GO:0009408; P:response to heat; IEA:InterPro
GO:0006986; P:response to unfolded protein; TAS:ProtInc
GO:0030317; P:sperm motility; IEA:Ensembl
GO:0007283; P:spermatogenesis; IEA:Ensembl

Interpro

InterPro; IPR012724; DnaJ
InterPro; IPR002939; DnaJ_C
InterPro; IPR001623; DnaJ_domain
InterPro; IPR018253; DnaJ_domain_CS
InterPro; IPR008971; HSP40/DnaJ_pept-bd
InterPro; IPR001305; HSP_DnaJ_Cys-rich_dom

Pfam

Pfam; PF01556; CTDII;
Pfam; PF00226; DnaJ;
Pfam; PF00684; DnaJ_CXXCXGXG;

SMART

SMART; SM00271; DnaJ;

PROSITE

PROSITE; PS00636; DNAJ_1;
PROSITE; PS50076; DNAJ_2;
PROSITE; PS51188; ZF_CR;

PRINTS

PRINTS; PR00625; JDOMAIN;