LipidDB ID

LipidDB-9606-00538

Entry Name

UniProt Accession

P35813; B5BU11; J3KNM0; O75551;

Theoretical PI

5.19

Molecular Weight

42447.74

Genbank Protein ID

AAB21784.1; AAC28354.1; BAG70061.1; EAW80774.1; AAH26691.1; AAH63243.1;

Genbank Nucleotide ID

S87759; AF070670; AK097843; AB451247; AL132778; AL157756; CH471061; BC026691; BC063243;

Protein Name

Protein phosphatase 1A

Protein Synonyms/Alias

3.1.3.16; Protein phosphatase 2C isoform alpha; PP2C-alpha; Protein phosphatase IA;

Gene Name

PPM1A

Gene Synonyms/Alias

PPPM1A;

Created Date

01-JUN-1994

Organism

Homo sapiens (Human)

NCBI Taxa ID

9606

Reference

[1] Suzuki T, Moriya K, Nagatoshi K, Ota Y, Ezure T, Ando E, Tsunasawa S, UtsumiT. Strategy for comprehensive identification of human N-myristoylated proteinsusing an insect cell-free protein synthesis system. Proteomics. 2010May;10(9):1780-93. doi: 10.1002/pmic.200900783.[PMID:20213681]

Functional Description

Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. Dephosphorylates PRKAA1 and PRKAA2. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB.

Sequence Annotation

Metal binding site: 60 60 Manganese 1.
Metal binding site: 60 60 Manganese 2.
Metal binding site: 61 61 Manganese 1; via carbonyl oxygen.
Metal binding site: 239 239 Manganese 2.
Metal binding site: 282 282 Manganese 2.
Modified residue: 375 375 Phosphoserine.

Protein Length

382 AA.

Protein Sequence
(Canonical)

FASTA
(Canonical)

Gene Ontology

GO:0005829; C:cytosol; ISS:UniProtKB
GO:0016020; C:membrane; ISS:UniProtKB
GO:0043005; C:neuron projection; IEA:Ensembl
GO:0005654; C:nucleoplasm; TAS:Reactome
GO:0005634; C:nucleus; IDA:UniProtKB
GO:0005891; C:voltage-gated calcium channel complex; IEA:Ensembl
GO:0033192; F:calmodulin-dependent protein phosphatase activity; IDA:UniProtKB
GO:0000287; F:magnesium ion binding; IEA:InterPro
GO:0030145; F:manganese ion binding; IEA:InterPro
GO:0004722; F:protein serine/threonine phosphatase activity; IDA:UniProtKB
GO:0070412; F:R-SMAD binding; IPI:UniProtKB
GO:0004871; F:signal transducer activity; IMP:UniProtKB
GO:0007050; P:cell cycle arrest; TAS:Reactome
GO:0016311; P:dephosphorylation; IDA:BHF-UCL
GO:0010467; P:gene expression; TAS:Reactome
GO:0008286; P:insulin receptor signaling pathway; TAS:Reactome
GO:0006499; P:N-terminal protein myristoylation; ISS:UniProtKB
GO:0043124; P:negative regulation of I-kappaB kinase/NF-kappaB signaling; IMP:UniProtKB
GO:0042347; P:negative regulation of NF-kappaB import into nucleus; IMP:UniProtKB
GO:0010991; P:negative regulation of SMAD protein complex assembly; IDA:UniProtKB
GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; TAS:Reactome
GO:0030512; P:negative regulation of transforming growth factor beta receptor signaling pathway; IDA:UniProtKB
GO:0035970; P:peptidyl-threonine dephosphorylation; IDA:UniProtKB
GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB signaling; IMP:UniProtKB
GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:BHF-UCL
GO:0030177; P:positive regulation of Wnt signaling pathway; IDA:BHF-UCL
GO:0006470; P:protein dephosphorylation; IMP:UniProtKB
GO:0006367; P:transcription initiation from RNA polymerase II promoter; TAS:Reactome
GO:0006351; P:transcription, DNA-templated; TAS:Reactome
GO:0007179; P:transforming growth factor beta receptor signaling pathway; TAS:Reactome
GO:0016055; P:Wnt signaling pathway; IDA:BHF-UCL

Interpro

InterPro; IPR001932; PP2C-like_dom
InterPro; IPR012911; PP2C_C
InterPro; IPR000222; PP2C_Mn2_Asp60_BS
InterPro; IPR015655; Protein_Pase_2C

Pfam

Pfam; PF00481; PP2C;
Pfam; PF07830; PP2C_C;

SMART

SMART; SM00331; PP2C_SIG;
SMART; SM00332; PP2Cc;

PROSITE

PROSITE; PS01032; PP2C;

PRINTS