LipidDB ID

LipidDB-9606-00484

Entry Name

UniProt Accession

Q13177; Q13154; Q6ISC3;

Theoretical PI

5.69

Molecular Weight

58042.61

Genbank Protein ID

AAA65442.1; AAH69613.1; AAA75468.1;

Genbank Nucleotide ID

U24153; BC069613; U25975;

Protein Name

PAK-2p34

Protein Synonyms/Alias

2.7.11.1; Gamma-PAK; PAK65; S6/H4 kinase; p21-activated kinase 2; PAK-2; p58; p27; p34; C-t-PAK2;

Gene Name

PAK2

Gene Synonyms/Alias

Created Date

01-NOV-1997

Organism

Homo sapiens (Human)

NCBI Taxa ID

9606

Reference

[1] Vilas GL, Corvi MM, Plummer GJ, Seime AM, Lambkin GR, Berthiaume LG.Posttranslational myristoylation of caspase-activated p21-activated proteinkinase 2 (PAK2) potentiates late apoptotic events. Proc Natl Acad Sci U S A. 2006Apr 25;103(17):6542-7. Epub 2006 Apr 14.[PMID:16617111]

Functional Description

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Full-length PAK2 stimulates cell survival and cell growth. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Phosphorylates JUN and plays an important role in EGF- induced cell proliferation. Phosphorylates many other substrates including histone H4 to promote assembly of H3.3 and H4 into nucleosomes, BAD, ribosomal protein S6, or MBP. Additionally, associates with ARHGEF7 and GIT1 to perform kinase-independent functions such as spindle orientation control during mitosis. On the other hand, apoptotic stimuli such as DNA damage lead to caspase-mediated cleavage of PAK2, generating PAK-2p34, an active p34 fragment that translocates to the nucleus and promotes cellular apoptosis involving the JNK signaling pathway. Caspase- activated PAK2 phosphorylates MKNK1 and reduces cellular translation.

Sequence Annotation

Domain: 74 87 CRIB.
Domain: 249 499 Protein kinase.
Nucleotide-binding: 255 263 ATP.
Region: 69 137 Autoregulatory region.
Region: 69 112 GTPase-binding.
Motif: 245 251 Nuclear localization signal.
Active site: 367 367 Proton acceptor.
Binding site: 278 278 ATP.
Functional site: 212 213 Cleavage; by caspase-3 or caspase-3-likeproteases.
Modified residue: 2 2 N-acetylserine.
Modified residue: 2 2 Phosphoserine.
Modified residue: 58 58 Phosphoserine.
Modified residue: 62 62 N6-acetyllysine.
Modified residue: 128 128 N6-acetyllysine.
Modified residue: 141 141 Phosphoserine.
Modified residue: 169 169 Phosphothreonine.
Modified residue: 197 197 Phosphoserine.
Modified residue: 402 402 Phosphothreonine; by autocatalysis.

Protein Length

524 AA.

Protein Sequence
(Canonical)

FASTA
(Canonical)

Gene Ontology

GO:0005737; C:cytoplasm; IDA:HPA
GO:0005829; C:cytosol; TAS:Reactome
GO:0005634; C:nucleus; IDA:HPA
GO:0005886; C:plasma membrane; TAS:Reactome
GO:0005524; F:ATP binding; IEA:UniProtKB-KW
GO:0042802; F:identical protein binding; IPI:IntAct
GO:0004672; F:protein kinase activity; TAS:ProtInc
GO:0019901; F:protein kinase binding; IPI:BHF-UCL
GO:0004674; F:protein serine/threonine kinase activity; IDA:BHF-UCL
GO:0030296; F:protein tyrosine kinase activator activity; IDA:BHF-UCL
GO:0006915; P:apoptotic process; TAS:Reactome
GO:0007411; P:axon guidance; TAS:Reactome
GO:0006921; P:cellular component disassembly involved in execution phase of apoptosis; TAS:Reactome
GO:0038095; P:Fc-epsilon receptor signaling pathway; TAS:Reactome
GO:0045087; P:innate immune response; TAS:Reactome
GO:0043066; P:negative regulation of apoptotic process; IMP:UniProtKB
GO:2001271; P:negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis; IDA:UniProtKB
GO:0006469; P:negative regulation of protein kinase activity; TAS:ProtInc
GO:0018105; P:peptidyl-serine phosphorylation; IDA:BHF-UCL
GO:0016310; P:phosphorylation; IDA:UniProtKB
GO:2001238; P:positive regulation of extrinsic apoptotic signaling pathway; IMP:UniProtKB
GO:0050731; P:positive regulation of peptidyl-tyrosine phosphorylation; IDA:BHF-UCL
GO:0061098; P:positive regulation of protein tyrosine kinase activity; IDA:GOC
GO:0046777; P:protein autophosphorylation; IDA:MGI
GO:0006468; P:protein phosphorylation; IDA:MGI
GO:0042981; P:regulation of apoptotic process; TAS:Reactome
GO:0050690; P:regulation of defense response to virus by virus; TAS:Reactome
GO:0040008; P:regulation of growth; IEA:UniProtKB-KW
GO:0007165; P:signal transduction; TAS:ProtInc
GO:0031295; P:T cell costimulation; TAS:Reactome
GO:0050852; P:T cell receptor signaling pathway; TAS:Reactome
GO:0016032; P:viral process; TAS:Reactome

Interpro

InterPro; IPR000095; CRIB_dom
InterPro; IPR011009; Kinase-like_dom
InterPro; IPR000719; Prot_kinase_dom
InterPro; IPR017441; Protein_kinase_ATP_BS
InterPro; IPR002290; Ser/Thr_dual-sp_kinase
InterPro; IPR008271; Ser/Thr_kinase_AS

Pfam

Pfam; PF00786; PBD;
Pfam; PF00069; Pkinase;

SMART

SMART; SM00285; PBD;
SMART; SM00220; S_TKc;

PROSITE

PROSITE; PS50108; CRIB;
PROSITE; PS00107; PROTEIN_KINASE_ATP;
PROSITE; PS50011; PROTEIN_KINASE_DOM;
PROSITE; PS00108; PROTEIN_KINASE_ST;

PRINTS