LipidDB ID

LipidDB-9606-00356

Entry Name

UniProt Accession

Q92900; O00239; O43343; Q86Z25; Q92842;

Theoretical PI

6.18

Molecular Weight

124345.27

Genbank Protein ID

AAC50771.1; AAC51140.1; BAA19664.2; AAC26788.1; AAB94785.1; AAH39817.1;

Genbank Nucleotide ID

U65533; U59323; D86988; AF074016; AC003972; BC039817;

Protein Name

Regulator of nonsense transcripts 1

Protein Synonyms/Alias

3.6.4.-; ATP-dependent helicase RENT1; Nonsense mRNA reducing factor 1; NORF1; Up-frameshift suppressor 1 homolog; hUpf1;

Gene Name

UPF1

Gene Synonyms/Alias

KIAA0221; RENT1;

Created Date

18-OCT-2001

Organism

Homo sapiens (Human)

NCBI Taxa ID

9606

Reference

[1] Predicted from GPS-Lipid

Functional Description

RNA-dependent helicase and ATPase required for nonsense- mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation; its phosphorylation appears to be a key step in NMD. Recruited by release factors to stalled ribosomes together with the SMG1C protein kinase complex to form the transient SURF (SMG1-UPF1-eRF1- eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) (located 50-55 or more nucleotides downstream from the termination codon) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Phosphorylated UPF1 is recognized by EST1B/SMG5, SMG6 and SMG7 which are thought to provide a link to the mRNA degradation machinery involving exonucleolytic and endonucleolytic pathways, and to serve as adapters to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation and allowing the recycling of NMD factors. UPF1 can also activate NMD without UPF2 or UPF3, and in the absence of the NMD-enhancing downstream EJC indicative for alternative NMD pathways. Plays a role in replication-dependent histone mRNA degradation at the end of phase S; the function is independent of UPF2. For the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. The ATPase activity of UPF1 is required for disassembly of mRNPs undergoing NMD. Essential for embryonic viability.

Sequence Annotation

Nucleotide-binding: 506 510 ATP.
Region: 1 415 Sufficient for interaction with RENT2.
Motif: 1089 1090 [ST]-Q motif 1.
Motif: 1107 1108 [ST]-Q motif 2.
Binding site: 486 486 ATP.
Binding site: 676 676 ATP.
Binding site: 713 713 ATP.
Binding site: 844 844 ATP.
Modified residue: 1089 1089 Phosphoserine.
Modified residue: 1107 1107 Phosphoserine.
Modified residue: 1110 1110 Phosphoserine.
Modified residue: 1127 1127 Phosphoserine.

Protein Length

1129 AA.

Protein Sequence
(Canonical)

FASTA
(Canonical)

Gene Ontology

GO:0000785; C:chromatin; IDA:HGNC
GO:0005737; C:cytoplasm; NAS:UniProtKB
GO:0005829; C:cytosol; TAS:Reactome
GO:0035145; C:exon-exon junction complex; IDA:UniProtKB
GO:0005634; C:nucleus; IDA:UniProtKB
GO:0044530; C:supraspliceosomal complex; IDA:UniProtKB
GO:0005524; F:ATP binding; IEA:UniProtKB-KW
GO:0004004; F:ATP-dependent RNA helicase activity; IDA:UniProtKB
GO:0003682; F:chromatin binding; IDA:HGNC
GO:0003677; F:DNA binding; IEA:InterPro
GO:0004386; F:helicase activity; NAS:UniProtKB
GO:0044822; F:poly(A) RNA binding; IDA:UniProtKB
GO:0003723; F:RNA binding; NAS:UniProtKB
GO:0008270; F:zinc ion binding; IEA:InterPro
GO:0006200; P:ATP catabolic process; IDA:GOC
GO:0006281; P:DNA repair; IDA:HGNC
GO:0006260; P:DNA replication; IMP:HGNC
GO:0009048; P:dosage compensation by inactivation of X chromosome; IEA:Ensembl
GO:0010467; P:gene expression; TAS:Reactome
GO:0071044; P:histone mRNA catabolic process; IMP:UniProtKB
GO:0006406; P:mRNA export from nucleus; TAS:HGNC
GO:0016071; P:mRNA metabolic process; TAS:Reactome
GO:0000956; P:nuclear-transcribed mRNA catabolic process; IMP:UniProt
GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IDA:UniProtKB
GO:0006449; P:regulation of translational termination; IMP:UniProtKB
GO:0016070; P:RNA metabolic process; TAS:Reactome

Interpro

InterPro; IPR027417; P-loop_NTPase
InterPro; IPR018999; RNA-helicase_UPF1_UPF2-interct

Pfam

Pfam; PF09416; UPF1_Zn_bind;

SMART

PROSITE

PRINTS