LipidDB ID

LipidDB-9606-00255

Entry Name

UniProt Accession

Q96BS2; F5H1Y5; Q9NWT9;

Theoretical PI

4.84

Molecular Weight

24749.88

Genbank Protein ID

AAL35615.1; BAA91288.1; EAW98101.1; AAH15221.1;

Genbank Nucleotide ID

AF443207; AK000614; AC026368; CH471054; BC015221; BQ949772;

Protein Name

Calcineurin B homologous protein 3

Protein Synonyms/Alias

Tescalcin; TSC;

Gene Name

TESC

Gene Synonyms/Alias

CHP3;

Created Date

02-MAY-2002

Organism

Homo sapiens (Human)

NCBI Taxa ID

9606

Reference

[1] Gutierrez-Ford C, Levay K, Gomes AV, Perera EM, Som T, Kim YM, Benovic JL,Berkovitz GD, Slepak VZ. Characterization of tescalcin, a novel EF-hand proteinwith a single Ca2+-binding site: metal-binding properties, localization intissues and cells, and effect on calcineurin. Biochemistry. 2003 Dec16;42(49):14553-65.[PMID:14661968]
[2] Zaun HC, Shrier A, Orlowski J. N-myristoylation and Ca2+ binding ofcalcineurin B homologous protein CHP3 are required to enhance Na+/H+ exchangerNHE1 half-life and activity at the plasma membrane. J Biol Chem. 2012 Oct26;287(44):36883-95. doi: 10.1074/jbc.M112.394700. Epub 2012 Sep 14.[PMID:22984264]

Functional Description

Functions as an integral cofactor in cell pH regulation by controlling plasma membrane-type Na(+)/H(+) exchange activity. Promotes the maturation, transport, cell surface stability and exchange activity of SLC9A1/NHE1 at the plasma membrane. Promotes the induction of hematopoietic stem cell differentiation toward megakaryocytic lineage. Essential for the coupling of ERK cascade activation with the expression of ETS family genes in megakaryocytic differentiation. Also involved in granulocytic differentiation in a ERK-dependent manner. Inhibits the phosphatase activity of calcineurin.

Sequence Annotation

Domain: 110 145 EF-hand.

Protein Length

214 AA.

Protein Sequence
(Canonical)

FASTA
(Canonical)

Gene Ontology

GO:0005737; C:cytoplasm; IDA:UniProtKB
GO:0030027; C:lamellipodium; IDA:UniProtKB
GO:0005634; C:nucleus; IDA:UniProtKB
GO:0005886; C:plasma membrane; IDA:UniProtKB
GO:0001726; C:ruffle; ISS:UniProtKB
GO:0005509; F:calcium ion binding; IDA:UniProtKB
GO:0000287; F:magnesium ion binding; ISS:UniProtKB
GO:0019212; F:phosphatase inhibitor activity; ISS:UniProtKB
GO:0042803; F:protein homodimerization activity; ISS:UniProtKB
GO:0004860; F:protein kinase inhibitor activity; IEA:UniProtKB-KW
GO:0030154; P:cell differentiation; IEA:UniProtKB-KW
GO:0071300; P:cellular response to retinoic acid; IDA:UniProtKB
GO:0043086; P:negative regulation of catalytic activity; ISS:GOC
GO:0008285; P:negative regulation of cell proliferation; IMP:UniProtKB
GO:0010628; P:positive regulation of gene expression; IMP:UniProtKB
GO:0030854; P:positive regulation of granulocyte differentiation; IDA:UniProtKB
GO:0045654; P:positive regulation of megakaryocyte differentiation; IMP:UniProtKB
GO:0032417; P:positive regulation of sodium:proton antiporter activity; IDA:UniProtKB
GO:0045893; P:positive regulation of transcription, DNA-templated; IMP:UniProtKB
GO:0051604; P:protein maturation; IDA:UniProtKB
GO:0050821; P:protein stabilization; IDA:UniProtKB
GO:0072661; P:protein targeting to plasma membrane; IDA:UniProtKB
GO:0033628; P:regulation of cell adhesion mediated by integrin; IMP:UniProtKB

Interpro

InterPro; IPR011992; EF-hand-dom_pair
InterPro; IPR018247; EF_Hand_1_Ca_BS
InterPro; IPR002048; EF_hand_dom

Pfam

Pfam; PF13405; EF-hand_6;

SMART

PROSITE

PROSITE; PS00018; EF_HAND_1;
PROSITE; PS50222; EF_HAND_2;

PRINTS