| Tag |
Content |
LipidDB ID |
LipidDB-928306-01085 |
Entry Name |
|
UniProt Accession |
|
Theoretical PI |
8.6 |
Molecular Weight |
60858.3 |
Genbank Protein ID |
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Genbank Nucleotide ID |
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Protein Name |
Nucleocapsid protein p10 |
Protein Synonyms/Alias |
Pr65gag; Core polyprotein; MA; pp12; CA; NC-gag; |
Gene Name |
gag |
Gene Synonyms/Alias |
|
Created Date |
21-JUL-1986 |
| Lipid Modification Sites |
|---|
| Position |
Sequence Form |
Peptide |
References |
Modification Type |
2 | Canonical | ******MGQTVTTPL | [1][2] | N-Myristoylation |
|
Organism |
Moloney murine leukemia virus (isolate Shinnick) (MoMLV) |
NCBI Taxa ID |
928306 |
Reference |
[1] Jacobs E, Gheysen D, Thines D, Francotte M, de Wilde M. The HIV-1 Gagprecursor Pr55gag synthesized in yeast is myristoylated and targeted to theplasma membrane. Gene. 1989 Jun 30;79(1):71-81.[ PMID:2673935]
[2] Henderson LE, Krutzsch HC, Oroszlan S. Myristyl amino-terminal acylation ofmurine retrovirus proteins: an unusual post-translational proteins modification. Proc Natl Acad Sci U S A. 1983 Jan;80(2):339-43.[ PMID:6340098]
|
Functional Description |
Gag polyprotein plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably link the viral protein to the host ESCRT pathway and facilitate release. |
Sequence Annotation |
Region: 345 393 Interaction with mouse PIAS4.
Region: 430 435 Interaction with mouse UBE2I.
Motif: 111 114 PTAP/PSAP motif.
Motif: 130 134 LYPX(n)L motif.
Motif: 162 165 PPXY motif.
Functional site: 131 132 Cleavage; by viral protease p14.
Functional site: 215 216 Cleavage; by viral protease p14.
Functional site: 478 479 Cleavage; by viral protease p14.
Modified residue: 192 192 Phosphoserine; by host.
|
Protein Length |
538 AA. |
Protein Sequence
(Canonical) |
MGQTVTTPLS LTLGHWKDVE RIAHNQSVDV KKRRWVTFCS AEWPTFNVGW PRDGTFNRDL 60
ITQVKIKVFS PGPHGHPDQV PYIVTWEALA FDPPPWVKPF VHPKPPPPLP PSAPSLPLEP 120
PRSTPPRSSL YPALTPSLGA KPKPQVLSDS GGPLIDLLTE DPPPYRDPRP PPSDRDGNGG 180
EATPAGEAPD PSPMASRLRG RREPPVADST TSQAFPLRAG GNGQLQYWPF SSSDLYNWKN 240
NNPSFSEDPG KLTALIESVL ITHQPTWDDC QQLLGTLLTG EEKQRVLLEA RKAVRGDDGR 300
PTQLPNEVDA AFPLERPDWD YTTQAGRNHL VHYRQLLLAG LQNAGRSPTN LAKVKGITQG 360
PNESPSAFLE RLKEAYRRYT PYDPEDPGQE TNVSMSFIWQ SAPDIGRKLE RLEDLKNKTL 420
GDLVREAEKI FNKRETPEER EERIRRETEE KEERRRTEDE QKEKERDRRR HREMSKLLAT 480
VVSGQKQDRQ GGERRRSQLD RDQCAYCKEK GHWAKDCPKK PRGPRGPRPQ TSLLTLDD 538
|
FASTA
(Canonical) |
>LipidDB-928306-01085|P03332
MGQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTFNVGWPRDGTFNRDL
ITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKPPPPLPPSAPSLPLEP
PRSTPPRSSLYPALTPSLGAKPKPQVLSDSGGPLIDLLTEDPPPYRDPRPPPSDRDGNGG
EATPAGEAPDPSPMASRLRGRREPPVADSTTSQAFPLRAGGNGQLQYWPFSSSDLYNWKN
NNPSFSEDPGKLTALIESVLITHQPTWDDCQQLLGTLLTGEEKQRVLLEARKAVRGDDGR
PTQLPNEVDAAFPLERPDWDYTTQAGRNHLVHYRQLLLAGLQNAGRSPTNLAKVKGITQG
PNESPSAFLERLKEAYRRYTPYDPEDPGQETNVSMSFIWQSAPDIGRKLERLEDLKNKTL
GDLVREAEKIFNKRETPEEREERIRRETEEKEERRRTEDEQKEKERDRRRHREMSKLLAT
VVSGQKQDRQGGERRRSQLDRDQCAYCKEKGHWAKDCPKKPRGPRGPRPQTSLLTLDD
|
Gene Ontology |
GO:0044174; C:host cell endosome; IEA:UniProtKB-KW GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-KW GO:0016020; C:membrane; IEA:UniProtKB-KW GO:0019013; C:viral nucleocapsid; IEA:UniProtKB-KW GO:0003723; F:RNA binding; IEA:UniProtKB-KW GO:0039660; F:structural constituent of virion; IEA:UniProtKB-KW GO:0008270; F:zinc ion binding; IEA:InterPro GO:0039702; P:viral budding via host ESCRT complex; IEA:UniProtKB-KW GO:0019076; P:viral release from host cell; IEA:UniProtKB-KW |
Interpro |
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Pfam |
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SMART |
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PROSITE |
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PRINTS |
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