LipidDB ID

LipidDB-8355-00725

Entry Name

UniProt Accession

P51469;

Theoretical PI

7.59

Molecular Weight

35697.78

Genbank Protein ID

AAA84422.1;

Genbank Nucleotide ID

U41753;

Protein Name

Glyceraldehyde-3-phosphate dehydrogenase

Protein Synonyms/Alias

GAPDH; 1.2.1.12; Peptidyl-cysteine S-nitrosylase GAPDH; 2.6.99.-;

Gene Name

gapdh

Gene Synonyms/Alias

gapd;

Created Date

01-OCT-1996

Organism

Xenopus laevis (African clawed frog)

NCBI Taxa ID

8355

Reference

[1] Yang J, Gibson B, Snider J, Jenkins CM, Han X, Gross RW. Submicromolarconcentrations of palmitoyl-CoA specifically thioesterify cysteine 244 inglyceraldehyde-3-phosphate dehydrogenase inhibiting enzyme activity: a novelmechanism potentially underlying fatty acid induced insulin resistance.Biochemistry. 2005 Sep 6;44(35):11903-12.[PMID:16128592]

Functional Description

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3- phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S- nitrosylation of nuclear target proteins (By similarity).

Sequence Annotation

Nucleotide-binding: 11 12 NAD.
Region: 149 151 Glyceraldehyde 3-phosphate binding.
Region: 209 210 Glyceraldehyde 3-phosphate binding.
Active site: 150 150 Nucleophile.
Binding site: 33 33 NAD.
Binding site: 78 78 NAD; via carbonyl oxygen.
Binding site: 180 180 Glyceraldehyde 3-phosphate.
Binding site: 232 232 Glyceraldehyde 3-phosphate.
Binding site: 314 314 NAD.
Functional site: 177 177 Activates thiol group during catalysis.
Modified residue: 150 150 S-nitrosocysteine.

Protein Length

333 AA.

Protein Sequence
(Canonical)

FASTA
(Canonical)

Gene Ontology

GO:0005737; C:cytoplasm; ISS:UniProtKB
GO:0005829; C:cytosol; ISS:UniProtKB
GO:0015630; C:microtubule cytoskeleton; ISS:UniProtKB
GO:0005634; C:nucleus; ISS:UniProtKB
GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; ISS:UniProtKB
GO:0008017; F:microtubule binding; ISS:UniProtKB
GO:0051287; F:NAD binding; IEA:InterPro
GO:0050661; F:NADP binding; IEA:InterPro
GO:0035605; F:peptidyl-cysteine S-nitrosylase activity; ISS:UniProtKB
GO:0006006; P:glucose metabolic process; IEA:InterPro
GO:0006096; P:glycolytic process; IEA:UniProtKB-UniPathway
GO:0000226; P:microtubule cytoskeleton organization; ISS:UniProtKB
GO:0051402; P:neuron apoptotic process; ISS:UniProtKB
GO:0035606; P:peptidyl-cysteine S-trans-nitrosylation; ISS:UniProtKB
GO:0050821; P:protein stabilization; ISS:UniProtKB

Interpro

InterPro; IPR020831; GlycerAld/Erythrose_P_DH
InterPro; IPR020830; GlycerAld_3-P_DH_AS
InterPro; IPR020829; GlycerAld_3-P_DH_cat
InterPro; IPR020828; GlycerAld_3-P_DH_NAD(P)-bd
InterPro; IPR006424; Glyceraldehyde-3-P_DH_1
InterPro; IPR016040; NAD(P)-bd_dom

Pfam

Pfam; PF02800; Gp_dh_C;
Pfam; PF00044; Gp_dh_N;

SMART

SMART; SM00846; Gp_dh_N;

PROSITE

PROSITE; PS00071; GAPDH;

PRINTS

PRINTS; PR00078; G3PDHDRGNASE;