Lipid Modification Database
Tag Content
LipidDB ID
LipidDB-7227-01324
Entry Name
UniProt Accession
Theoretical PI
7.47
Molecular Weight
26423.56
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
Protein spitz
Protein Synonyms/Alias
Gene Name
spi
Gene Synonyms/Alias
CG10334;
Created Date
01-NOV-1995
 Lipid Modification Sites 
 Position   Sequence Form   Peptide   References   Modification Type 
29
Canonical
HPWHVEACSSRTVPK
[1]
S-Palmitoylation
Organism
Drosophila melanogaster (Fruit fly)
NCBI Taxa ID
7227
Reference
[1] Miura GI, Buglino J, Alvarado D, Lemmon MA, Resh MD, Treisman JE.Palmitoylation of the EGFR ligand Spitz by Rasp increases Spitz activity byrestricting its diffusion. Dev Cell. 2006 Feb;10(2):167-76.[PMID:16459296]
Functional Description
Ligand for the EGF receptor (Gurken). Involved in a number of unrelated developmental choices, for example, dorsal- ventral axis formation, glial migration, sensory organ determination, and muscle development. It is required for photoreceptor determination.
Sequence Annotation
Topological domain: 29 143 Lumenal.
Transmembrane: 144 164 Helical.
Topological domain: 165 234 Cytoplasmic.
Domain: 78 122 EGF-like.
Protein Length
234 AA.
Protein Sequence
(Canonical)
MHSTMSVQHG LVALVLIGCL AHPWHVEACS SRTVPKPRSS ISSSMSGTAL PPTQAPVTSS  60
TTMRTTTTTT PRPNITFPTY KCPETFDAWY CLNDAHCFAV KIADLPVYSC ECAIGFMGQR  120
CEYKEIDNTY LPKRPRPMLE KASIASGAMC ALVFMLFVCL AFYLRFEQRA AKKAYELEQE  180
LQQEYDDDDG QCECCRNRCC PDGQEPVILE RKLPYHMRLE HALMSFAIRR SNKL        234
FASTA
(Canonical)
>LipidDB-7227-01324|Q01083
MHSTMSVQHGLVALVLIGCLAHPWHVEACSSRTVPKPRSSISSSMSGTALPPTQAPVTSS
TTMRTTTTTTPRPNITFPTYKCPETFDAWYCLNDAHCFAVKIADLPVYSCECAIGFMGQR
CEYKEIDNTYLPKRPRPMLEKASIASGAMCALVFMLFVCLAFYLRFEQRAAKKAYELEQE
LQQEYDDDDGQCECCRNRCCPDGQEPVILERKLPYHMRLEHALMSFAIRRSNKL
Gene Ontology
GO:0005783; C:endoplasmic reticulum; IDA:FlyBase
GO:0005576; C:extracellular region; NAS:FlyBase
GO:0005794; C:Golgi apparatus; IGI:FlyBase
GO:0016021; C:integral component of membrane; NAS:FlyBase
GO:0005886; C:plasma membrane; IEA:UniProtKB-KW
GO:0005154; F:epidermal growth factor receptor binding; NAS:FlyBase
GO:0048149; P:behavioral response to ethanol; IMP:FlyBase
GO:0007298; P:border follicle cell migration; IMP:FlyBase
GO:0046845; P:branched duct epithelial cell fate determination, open tracheal system; TAS:FlyBase
GO:0035225; P:determination of genital disc primordium; IMP:FlyBase
GO:0007173; P:epidermal growth factor receptor signaling pathway; NAS:FlyBase
GO:0061331; P:epithelial cell proliferation involved in Malpighian tubule morphogenesis; IMP:FlyBase
GO:0000086; P:G2/M transition of mitotic cell cycle; NAS:FlyBase
GO:0003015; P:heart process; IMP:FlyBase
GO:0007476; P:imaginal disc-derived wing morphogenesis; IMP:FlyBase
GO:0043066; P:negative regulation of apoptotic process; IMP:FlyBase
GO:0046673; P:negative regulation of compound eye retinal cell programmed cell death; NAS:FlyBase
GO:0022008; P:neurogenesis; IMP:FlyBase
GO:0007438; P:oenocyte development; IMP:FlyBase
GO:0008355; P:olfactory learning; IMP:FlyBase
GO:0007424; P:open tracheal system development; TAS:FlyBase
GO:0007422; P:peripheral nervous system development; IMP:FlyBase
GO:0008284; P:positive regulation of cell proliferation; IMP:FlyBase
GO:0045742; P:positive regulation of epidermal growth factor receptor signaling pathway; NAS:FlyBase
GO:0050769; P:positive regulation of neurogenesis; IMP:FlyBase
GO:0045470; P:R8 cell-mediated photoreceptor organization; NAS:FlyBase
GO:0007432; P:salivary gland boundary specification; NAS:FlyBase
GO:0016330; P:second mitotic wave involved in compound eye morphogenesis; NAS:FlyBase
GO:0035277; P:spiracle morphogenesis, open tracheal system; IMP:FlyBase
GO:0048865; P:stem cell fate commitment; IMP:FlyBase
GO:0007421; P:stomatogastric nervous system development; IMP:FlyBase
Interpro
InterPro; IPR000742; EG-like_dom
InterPro; IPR013032; EGF-like_CS
Pfam
Pfam; PF00008; EGF;
SMART
SMART; SM00181; EGF;
PROSITE
PROSITE; PS00022; EGF_1;
PROSITE; PS01186; EGF_2;
PROSITE; PS50026; EGF_3;
PRINTS