Lipid Modification Database
Tag Content
LipidDB ID
LipidDB-559292-01131
Entry Name
UniProt Accession
Theoretical PI
6.35
Molecular Weight
166372.12
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
Serine/threonine-protein kinase VPS15
Protein Synonyms/Alias
2.7.11.1; Golgi-retention defective mutant protein 8; Vacuolar protein sorting-associated protein 15;
Gene Name
VPS15
Gene Synonyms/Alias
GRD8; VAC4; VPL19; YBR097W; YBR0825;
Created Date
01-AUG-1991
 Lipid Modification Sites 
 Position   Sequence Form   Peptide   References   Modification Type 
2
Canonical
******MGAQLSLVV
[1]
N-Myristoylation
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
NCBI Taxa ID
559292
Reference
[1] Herman PK, Stack JH, Emr SD. A genetic and structural analysis of the yeastVps15 protein kinase: evidence for a direct role of Vps15p in vacuolar proteindelivery. EMBO J. 1991 Dec;10(13):4049-60.[PMID:1756716]
Functional Description
Serine/threonine-protein kinase required for cytoplasm to vacuole transport (Cvt) and autophagy as a part of the autophagy-specific VPS34 PI3-kinase complex I. This complex is essential to recruit the ATG8-phosphatidylinositol conjugate and the ATG12-ATG5 conjugate to the pre-autophagosomal structure. Is also involved in endosome-to-Golgi retrograde transport as part of the VPS34 PI3-kinase complex II. This second complex is required for the endosome-to-Golgi retrieval of PEP1 and KEX2, and the recruitment of VPS5 and VPS7, two components of the retromer complex, to endosomal membranes (probably through the synthesis of a specific pool of phosphatidylinositol 3-phosphate recruiting the retromer to the endosomes). By regulating VPS34 kinase activity, VPS15 appears to be essential for the efficient delivery of soluble hydrolases to the yeast vacuole.
Sequence Annotation
Domain: 27 300 Protein kinase.
Nucleotide-binding: 33 41 ATP.
Active site: 147 147 Proton acceptor.
Binding site: 54 54 ATP.
Protein Length
1454 AA.
Protein Sequence
(Canonical)
MGAQLSLVVQ ASPSIAIFSY IDVLEEVHYV SQLNSSRFLK TCKALDPNGE IVIKVFIKPK  60
DQYSLRPFLQ RIRAQSFKLG QLPHVLNYSK LIETNRAGYM IRQHLKNNLY DRLSLRPYLQ  120
DIELKFIAFQ LLNALKDIHN LNIVHGDIKT ENILVTSWNW CILTDFAAFI KPVYLPEDNP  180
GEFLFYFDTS KRRTCYLAPE RFNSKLYQDG KSNNGRLTKE MDIFSLGCVI AEIFAEGRPI  240
FNLSQLFKYK SNSYDVNREF LMEEMNSTDL RNLVLDMIQL DPSKRLSCDE LLNKYRGIFF  300
PDYFYTFIYD YFRNLVTMTT STPISDNTCT NSTLEDNVKL LDETTEKIYR DFSQICHCLD  360
FPLIKDGGEI GSDPPILESY KIEIEISRFL NTNLYFPQNY HLVLQQFTKV SEKIKSVKEE  420
CALLFISYLS HSIRSIVSTA TKLKNLELLA VFAQFVSDEN KIDRVVPYFV CCFEDSDQDV  480
QALSLLTLIQ VLTSVRKLNQ LNENIFVDYL LPRLKRLLIS NRQNTNYLRI VFANCLSDLA  540
IIINRFQEFT FAQHCNDNSM DNNTEIMESS TKYSAKLIQS VEDLTVSFLT DNDTYVKMAL  600
LQNILPLCKF FGRERTNDII LSHLITYLND KDPALRVSLI QTISGISILL GTVTLEQYIL  660
PLLIQTITDS EELVVISVLQ SLKSLFKTGL IRKKYYIDIS KTTSPLLLHP NNWIRQFTLM  720
IIIEIINKLS KAEVYCILYP IIRPFFEFDV EFNFKSMISC CKQPVSRSVY NLLCSWSVRA  780
SKSLFWKKII TNHVDSFGNN RIEFITKNYS SKNYGFNKRD TKSSSSLKGI KTSSTVYSHD  840
NKEIPLTAED RNWIDKFHII GLTEKDIWKI VALRGYVIRT ARVMAANPDF PYNNSNYRPL  900
VQNSPPNLNL TNIMPRNIFF DVEFAEESTS EGQDSNLENQ QIYKYDESEK DSNKLNINGS  960
KQLSTVMDIN GSLIFKNKSI ATTTSNLKNV FVQLEPTSYH MHSPNHGLKD NANVKPERKV  1020
VVSNSYEGDV ESIEKFLSTF KILPPLRDYK EFGPIQEIVR SPNMGNLRGK LIATLMENEP  1080
NSITSSAVSP GETPYLITGS DQGVIKIWNL KEIIVGEVYS SSLTYDCSST VTQITMIPNF  1140
DAFAVSSKDG QIIVLKVNHY QQESEVKFLN CECIRKINLK NFGKNEYAVR MRAFVNEEKS  1200
LLVALTNLSR VIIFDIRTLE RLQIIENSPR HGAVSSICID EECCVLILGT TRGIIDIWDI  1260
RFNVLIRSWS FGDHAPITHV EVCQFYGKNS VIVVGGSSKT FLTIWNFVKG HCQYAFINSD  1320
EQPSMEHFLP IEKGLEELNF CGIRSLNALS TISVSNDKIL LTDEATSSIV MFSLNELSSS  1380
KAVISPSRFS DVFIPTQVTA NLTMLLRKMK RTSTHSVDDS LYHHDIINSI STCEVDETPL  1440
LVACDNSGLI GIFQ                                                    1454
FASTA
(Canonical)
>LipidDB-559292-01131|P22219
MGAQLSLVVQASPSIAIFSYIDVLEEVHYVSQLNSSRFLKTCKALDPNGEIVIKVFIKPK
DQYSLRPFLQRIRAQSFKLGQLPHVLNYSKLIETNRAGYMIRQHLKNNLYDRLSLRPYLQ
DIELKFIAFQLLNALKDIHNLNIVHGDIKTENILVTSWNWCILTDFAAFIKPVYLPEDNP
GEFLFYFDTSKRRTCYLAPERFNSKLYQDGKSNNGRLTKEMDIFSLGCVIAEIFAEGRPI
FNLSQLFKYKSNSYDVNREFLMEEMNSTDLRNLVLDMIQLDPSKRLSCDELLNKYRGIFF
PDYFYTFIYDYFRNLVTMTTSTPISDNTCTNSTLEDNVKLLDETTEKIYRDFSQICHCLD
FPLIKDGGEIGSDPPILESYKIEIEISRFLNTNLYFPQNYHLVLQQFTKVSEKIKSVKEE
CALLFISYLSHSIRSIVSTATKLKNLELLAVFAQFVSDENKIDRVVPYFVCCFEDSDQDV
QALSLLTLIQVLTSVRKLNQLNENIFVDYLLPRLKRLLISNRQNTNYLRIVFANCLSDLA
IIINRFQEFTFAQHCNDNSMDNNTEIMESSTKYSAKLIQSVEDLTVSFLTDNDTYVKMAL
LQNILPLCKFFGRERTNDIILSHLITYLNDKDPALRVSLIQTISGISILLGTVTLEQYIL
PLLIQTITDSEELVVISVLQSLKSLFKTGLIRKKYYIDISKTTSPLLLHPNNWIRQFTLM
IIIEIINKLSKAEVYCILYPIIRPFFEFDVEFNFKSMISCCKQPVSRSVYNLLCSWSVRA
SKSLFWKKIITNHVDSFGNNRIEFITKNYSSKNYGFNKRDTKSSSSLKGIKTSSTVYSHD
NKEIPLTAEDRNWIDKFHIIGLTEKDIWKIVALRGYVIRTARVMAANPDFPYNNSNYRPL
VQNSPPNLNLTNIMPRNIFFDVEFAEESTSEGQDSNLENQQIYKYDESEKDSNKLNINGS
KQLSTVMDINGSLIFKNKSIATTTSNLKNVFVQLEPTSYHMHSPNHGLKDNANVKPERKV
VVSNSYEGDVESIEKFLSTFKILPPLRDYKEFGPIQEIVRSPNMGNLRGKLIATLMENEP
NSITSSAVSPGETPYLITGSDQGVIKIWNLKEIIVGEVYSSSLTYDCSSTVTQITMIPNF
DAFAVSSKDGQIIVLKVNHYQQESEVKFLNCECIRKINLKNFGKNEYAVRMRAFVNEEKS
LLVALTNLSRVIIFDIRTLERLQIIENSPRHGAVSSICIDEECCVLILGTTRGIIDIWDI
RFNVLIRSWSFGDHAPITHVEVCQFYGKNSVIVVGGSSKTFLTIWNFVKGHCQYAFINSD
EQPSMEHFLPIEKGLEELNFCGIRSLNALSTISVSNDKILLTDEATSSIVMFSLNELSSS
KAVISPSRFSDVFIPTQVTANLTMLLRKMKRTSTHSVDDSLYHHDIINSISTCEVDETPL
LVACDNSGLIGIFQ
Gene Ontology
GO:0005768; C:endosome; IEA:UniProtKB-KW
GO:0005794; C:Golgi apparatus; IEA:UniProtKB-KW
GO:0071561; C:nucleus-vacuole junction; IDA:SGD
GO:0034271; C:phosphatidylinositol 3-kinase complex I; IDA:SGD
GO:0034272; C:phosphatidylinositol 3-kinase complex II; IDA:SGD
GO:0005524; F:ATP binding; IEA:UniProtKB-KW
GO:0004674; F:protein serine/threonine kinase activity; IDA:SGD
GO:0043130; F:ubiquitin binding; IDA:SGD
GO:0048017; P:inositol lipid-mediated signaling; IMP:SGD
GO:0045324; P:late endosome to vacuole transport; IMP:SGD
GO:0016236; P:macroautophagy; IMP:SGD
GO:0030242; P:peroxisome degradation; IMP:SGD
GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IMP:SGD
GO:0006468; P:protein phosphorylation; IDA:SGD
GO:0045053; P:protein retention in Golgi apparatus; IMP:SGD
GO:0006623; P:protein targeting to vacuole; IMP:SGD
GO:0000011; P:vacuole inheritance; IMP:SGD
Interpro
InterPro; IPR011989; ARM-like
InterPro; IPR016024; ARM-type_fold
InterPro; IPR021133; HEAT_type_2
InterPro; IPR011009; Kinase-like_dom
InterPro; IPR000719; Prot_kinase_dom
InterPro; IPR008271; Ser/Thr_kinase_AS
InterPro; IPR015943; WD40/YVTN_repeat-like_dom
InterPro; IPR001680; WD40_repeat
InterPro; IPR017986; WD40_repeat_dom
Pfam
Pfam; PF00069; Pkinase;
SMART
SMART; SM00320; WD40;
PROSITE
PROSITE; PS50077; HEAT_REPEAT;
PROSITE; PS50011; PROTEIN_KINASE_DOM;
PROSITE; PS00108; PROTEIN_KINASE_ST;
PROSITE; PS50082; WD_REPEATS_2;
PROSITE; PS50294; WD_REPEATS_REGION;
PRINTS