LipidDB ID

LipidDB-559292-01017

Entry Name

UniProt Accession

P01123; D6VTJ2;

Theoretical PI

5.17

Molecular Weight

23214.25

Genbank Protein ID

CAA25036.1; BAA09201.1; AAS56793.1; DAA12402.1;

Genbank Nucleotide ID

X00209; D50617; AY558467; BK006940;

Protein Name

GTP-binding protein YPT1

Protein Synonyms/Alias

Protein YP2; Rab GTPase YPT1; Transport GTPase YPT1;

Gene Name

YPT1

Gene Synonyms/Alias

YP2; YFL038C;

Created Date

21-JUL-1986

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

NCBI Taxa ID

559292

Reference

[1] Yoo JS, Grabowski R, Xing L, Trepte HH, Schmitt HD, Gallwitz D. Functionalimplications of genetic interactions between genes encoding small GTPasesinvolved in vesicular transport in yeast. Mol Gen Genet. 1999 Feb;261(1):80-91.[PMID:10071213]

Functional Description

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. YPT1 regulates the trafficking of secretory vesicles from the endoplasmic reticulum (ER) to the Golgi. Vesicular transport depends on shuttling of YPT1 between membrane and cytosol by GDI1, probably by recycling it to its membrane of origin after a vesicle fusion event. Plays a role in the initial events of the autophagic vacuole development which take place at specialized regions of the endoplasmic reticulum. Also involved in the recycling of membrane proteins.

Sequence Annotation

Nucleotide-binding: 15 22 GTP.
Nucleotide-binding: 63 67 GTP.
Nucleotide-binding: 121 124 GTP.
Region: 63 80 Interaction with GDI1.
Region: 189 195 Interaction with GDI1.
Motif: 37 45 Effector region.
Modified residue: 1 1 N-acetylmethionine.
Modified residue: 172 172 Phosphoserine.
Modified residue: 174 174 Phosphoserine.

Protein Length

206 AA.

Protein Sequence
(Canonical)

FASTA
(Canonical)

Gene Ontology

GO:0031410; C:cytoplasmic vesicle; IDA:SGD
GO:0005789; C:endoplasmic reticulum membrane; IDA:SGD
GO:0000139; C:Golgi membrane; IDA:SGD
GO:0005795; C:Golgi stack; IDA:SGD
GO:0000407; C:pre-autophagosomal structure; IDA:SGD
GO:0005802; C:trans-Golgi network; IDA:SGD
GO:0005525; F:GTP binding; IEA:UniProtKB-KW
GO:0003924; F:GTPase activity; IDA:SGD
GO:0000149; F:SNARE binding; IDA:SGD
GO:0090114; P:COPII-coated vesicle budding; IMP:SGD
GO:0032258; P:CVT pathway; IMP:SGD
GO:0034498; P:early endosome to Golgi transport; IMP:SGD
GO:0032456; P:endocytic recycling; IMP:SGD
GO:0006888; P:ER to Golgi vesicle-mediated transport; IMP:SGD
GO:0048194; P:Golgi vesicle budding; IGI:SGD
GO:0048211; P:Golgi vesicle docking; IMP:SGD
GO:0006184; P:GTP catabolic process; IDA:GOC
GO:0016236; P:macroautophagy; IMP:SGD
GO:1990261; P:pre-mRNA catabolic process; IMP:SGD
GO:0006461; P:protein complex assembly; IDA:SGD
GO:1900101; P:regulation of endoplasmic reticulum unfolded protein response; IMP:SGD
GO:0006890; P:retrograde vesicle-mediated transport, Golgi to ER; IMP:SGD
GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro
GO:0035494; P:SNARE complex disassembly; IMP:SGD

Interpro

InterPro; IPR027417; P-loop_NTPase
InterPro; IPR005225; Small_GTP-bd_dom
InterPro; IPR001806; Small_GTPase
InterPro; IPR003579; Small_GTPase_Rab_type

Pfam

Pfam; PF00071; Ras;

SMART

SMART; SM00175; RAB;

PROSITE

PROSITE; PS51419; RAB;

PRINTS

PRINTS; PR00449; RASTRNSFRMNG;