| Tag |
Content |
LipidDB ID |
LipidDB-559292-00859 |
Entry Name |
|
UniProt Accession |
|
Theoretical PI |
5.24 |
Molecular Weight |
25637.93 |
Genbank Protein ID |
|
Genbank Nucleotide ID |
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Protein Name |
Vacuolar protein sorting-associated protein 20 |
Protein Synonyms/Alias |
Amino acid sensor-independent protein 10; Charged multivesicular body protein 6; ESCRT-III complex subunit VPS20; Vacuolar protein-targeting protein 20; |
Gene Name |
VPS20 |
Gene Synonyms/Alias |
ASI10; CHM6; VPT20; YMR077C; YM9582.02C; |
Created Date |
01-NOV-1997 |
| Lipid Modification Sites |
|---|
| Position |
Sequence Form |
Peptide |
References |
Modification Type |
2 | Canonical | ******MGQKSSKVH | [1] | N-Myristoylation |
|
Organism |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID |
559292 |
Reference |
[1] Ashrafi K, Farazi TA, Gordon JI. A role for Saccharomyces cerevisiae fattyacid activation protein 4 in regulating protein N-myristoylation during entryinto stationary phase. J Biol Chem. 1998 Oct 2;273(40):25864-74.[ PMID:9748261]
|
Functional Description |
Class E VPS protein implicated in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. The lumenal sequestrated membrane proteins will be targeted into the vacuole after fusion of the endosome with the vacuole. Acts a component of the ESCRT- III complex, which appears to be critical for late steps in MVB sorting, such as membrane invagination and final cargo sorting and recruitment of late-acting components of the sorting machinery. The MVB pathway requires the sequential function of ESCRT-O, -I,- II and -III complex assemblies. Required for the oligomerization of SNF7 into a membrane-associated filament. The VPS20-SNF7 subcomplex is responsible for the membrane association of the ESCRT-III complex. Also required for the RIM101 repressor proteolytic activation. |
Sequence Annotation |
|
Protein Length |
221 AA. |
Protein Sequence
(Canonical) |
MGQKSSKVHI TKTDRAILEV KRSKDEIHKF TRRTDNLILV EKSQLKDLIR KNPENYKSNM 60
KVRFLLKRIH YQEHLLQQAS DQLINLENMV STLEFKMVEK QFINGLKNGN EILKKLNKEF 120
SNVDELMDDV QDQIAYQNEI NETLSRSLVG TSNYEDDLDK ELDALESELN PEKMNNAKVA 180
NMPSTEGLPS LPQGEQTEQK EREEFATEER SDTKEPLALL S 221
|
FASTA
(Canonical) |
>LipidDB-559292-00859|Q04272
MGQKSSKVHITKTDRAILEVKRSKDEIHKFTRRTDNLILVEKSQLKDLIRKNPENYKSNM
KVRFLLKRIHYQEHLLQQASDQLINLENMVSTLEFKMVEKQFINGLKNGNEILKKLNKEF
SNVDELMDDVQDQIAYQNEINETLSRSLVGTSNYEDDLDKELDALESELNPEKMNNAKVA
NMPSTEGLPSLPQGEQTEQKEREEFATEERSDTKEPLALLS
|
Gene Ontology |
GO:0005737; C:cytoplasm; IDA:SGD GO:0000815; C:ESCRT III complex; IDA:SGD GO:0005773; C:vacuole; IEA:UniProtKB-KW GO:0070676; P:intralumenal vesicle formation; IMP:SGD GO:0045324; P:late endosome to vacuole transport; IMP:SGD GO:0015031; P:protein transport; IEA:UniProtKB-KW GO:0043162; P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IC:SGD |
Interpro |
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Pfam |
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SMART |
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PROSITE |
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PRINTS |
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