LipidDB ID

LipidDB-392810-00062

Entry Name

UniProt Accession

P03492; Q76V61;

Theoretical PI

5.1

Molecular Weight

11225.84

Genbank Protein ID

CAA29335.1; AAA43257.1; AAA43259.1;

Genbank Nucleotide ID

X05905; M55474; M55475;

Protein Name

Matrix protein 2

Protein Synonyms/Alias

Proton channel protein M2;

Gene Name

M

Gene Synonyms/Alias

Created Date

21-JUL-1986

Organism

Influenza A virus (strain A/Fowl plague virus/Rostock/8/1934 H7N1)

NCBI Taxa ID

392810

Reference

[1] Sugrue RJ, Belshe RB, Hay AJ. Palmitoylation of the influenza A virus M2protein. Virology. 1990 Nov;179(1):51-6.[PMID:2219738]

Functional Description

Forms a proton-selective ion channel that is necessary for the efficient release of the viral genome during virus entry. After attaching to the cell surface, the virion enters the cell by endocytosis. Acidification of the endosome triggers M2 ion channel activity. The influx of protons into virion interior is believed to disrupt interactions between the viral ribonucleoprotein (RNP), matrix protein 1 (M1), and lipid bilayers, thereby freeing the viral genome from interaction with viral proteins and enabling RNA segments to migrate to the host cell nucleus, where influenza virus RNA transcription and replication occur. Also plays a role in viral proteins secretory pathway. Elevates the intravesicular pH of normally acidic compartments, such as trans-Golgi network, preventing newly formed hemagglutinin from premature switching to the fusion-active conformation.

Sequence Annotation

Topological domain: 1 22 Virion surface.
Transmembrane: 23 43 Helical; Signal-anchor for type IIImembrane protein.
Topological domain: 44 97 Intravirion.
Functional site: 37 37 Essential for channel activity, seems tobe protonated during channel activation,and may play a role in the channel gatingand selectivity.
Functional site: 41 41 Seems to be involved in pH gating.
Modified residue: 64 64 Phosphoserine; by host.
Modified residue: 82 82 Phosphoserine; by host.

Protein Length

97 AA.

Protein Sequence
(Canonical)

FASTA
(Canonical)

Gene Ontology

GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-KW
GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW
GO:0044385; C:integral to membrane of host cell; IEA:UniProtKB-KW
GO:0055036; C:virion membrane; IEA:InterPro
GO:0015078; F:hydrogen ion transmembrane transporter activity; IEA:InterPro
GO:0005216; F:ion channel activity; IEA:UniProtKB-KW
GO:0039707; P:pore formation by virus in membrane of host cell; IEA:UniProtKB-KW
GO:0051259; P:protein oligomerization; IEA:UniProtKB-KW
GO:0039521; P:suppression by virus of host autophagy; IEA:UniProtKB-KW

Interpro

InterPro; IPR002089; Flu_M2

Pfam

Pfam; PF00599; Flu_M2;

SMART

PROSITE

PRINTS