LipidDB ID

LipidDB-381518-01056

Entry Name

UniProt Accession

P05780; Q67182;

Theoretical PI

5.12

Molecular Weight

11312.97

Genbank Protein ID

CAA30883.1; CAA30885.1; AAA43274.1; AAA91322.1; AAA91324.1;

Genbank Nucleotide ID

X08088; X08089; M23922; L25814; L25818;

Protein Name

Matrix protein 2

Protein Synonyms/Alias

Proton channel protein M2;

Gene Name

M

Gene Synonyms/Alias

Created Date

01-NOV-1988

Organism

Influenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A viru (strain A/WS/1933 H1N1))

NCBI Taxa ID

381518

Reference

[1] Grantham ML, Wu WH, Lalime EN, Lorenzo ME, Klein SL, Pekosz A. Palmitoylation of the influenza A virus M2 protein is not required for virus replication invitro but contributes to virus virulence. J Virol. 2009 Sep;83(17):8655-61. doi: 10.1128/JVI.01129-09. Epub 2009 Jun 24.[PMID:19553312]

Functional Description

Forms a proton-selective ion channel that is necessary for the efficient release of the viral genome during virus entry. After attaching to the cell surface, the virion enters the cell by endocytosis. Acidification of the endosome triggers M2 ion channel activity. The influx of protons into virion interior is believed to disrupt interactions between the viral ribonucleoprotein (RNP), matrix protein 1 (M1), and lipid bilayers, thereby freeing the viral genome from interaction with viral proteins and enabling RNA segments to migrate to the host cell nucleus, where influenza virus RNA transcription and replication occur. Also plays a role in viral proteins secretory pathway. Elevates the intravesicular pH of normally acidic compartments, such as trans-Golgi network, preventing newly formed hemagglutinin from premature switching to the fusion-active conformation (By similarity).

Sequence Annotation

Topological domain: 1 22 Virion surface.
Transmembrane: 23 43 Helical; Signal-anchor for type IIImembrane protein.
Topological domain: 44 97 Intravirion.
Functional site: 37 37 Essential for channel activity, seems tobe protonated during channel activation,and may play a role in the channel gatingand selectivity.
Functional site: 41 41 Seems to be involved in pH gating.
Modified residue: 64 64 Phosphoserine; by host.

Protein Length

97 AA.

Protein Sequence
(Canonical)

FASTA
(Canonical)

Gene Ontology

GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-KW
GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW
GO:0044385; C:integral to membrane of host cell; IEA:UniProtKB-KW
GO:0055036; C:virion membrane; IEA:InterPro
GO:0015078; F:hydrogen ion transmembrane transporter activity; IEA:InterPro
GO:0005216; F:ion channel activity; IEA:UniProtKB-KW
GO:0039707; P:pore formation by virus in membrane of host cell; IEA:UniProtKB-KW
GO:0051259; P:protein oligomerization; IEA:UniProtKB-KW
GO:0039521; P:suppression by virus of host autophagy; IEA:UniProtKB-KW

Interpro

InterPro; IPR002089; Flu_M2

Pfam

Pfam; PF00599; Flu_M2;

SMART

PROSITE

PRINTS