LipidDB-3702-01275

Lipid Modification Database

Tag

Content

LipidDB ID

LipidDB-3702-01275

Entry Name

UniProt Accession

Q9FL28; Q0WVN3; Q84WF4;

Theoretical PI

5.53

Molecular Weight

128824.08

Genbank Protein ID

BAB11088.1; AED95370.1; AAO41929.1; BAE98815.1;

Genbank Nucleotide ID

AB010698; CP002688; BT003880; AK226709;

Protein Name

LRR receptor-like serine/threonine-protein kinase FLS2

Protein Synonyms/Alias

2.7.11.1; Protein FLAGELLIN-SENSING 2; Protein FLAGELLIN-SENSITIVE 2;

Gene Name

FLS2

Gene Synonyms/Alias

At5g46330; MPL12.13; MPL12.8;

Created Date

18-MAR-2008

Lipid Modification Sites
Position	Sequence Form	Peptide	References	Modification Type
830	Canonical	LLVLILTCCKKKEKK	[1]	S-Palmitoylation
831	Canonical	LVLILTCCKKKEKKI	[1]	S-Palmitoylation

Organism

Arabidopsis thaliana (Mouse-ear cress)

NCBI Taxa ID

3702

Reference

[1] Hemsley PA, Weimar T, Lilley KS, Dupree P, Grierson CS. A proteomic approachidentifies many novel palmitoylated proteins in Arabidopsis. New Phytol. 2013Feb;197(3):805-14. doi: 10.1111/nph.12077. Epub 2012 Dec 17.[PMID:23252521]

Functional Description

Constitutes the pattern-recognition receptor (PPR) that determines the specific perception of flagellin (flg22), a potent elicitor of the defense response to pathogen-associated molecular patterns (PAMPs). Flagellin-binding to the receptor is the first step to initiate the innate immune MAP kinase signaling cascade (MEKK1, MKK4/MKK5 and MPK3/MPK6), resulting in enhanced resistance against pathogens. Binding to the effector AvrPto1 or to the phosphatase hopD2 from Pseudomonas syringae blocks the downstream plant immune response.

Sequence Annotation

Topological domain: 24 806 Extracellular.
Transmembrane: 807 827 Helical.
Topological domain: 828 1173 Cytoplasmic.
Domain: 870 1155 Protein kinase.
Nucleotide-binding: 876 884 ATP.
Active site: 997 997 Proton acceptor.
Binding site: 898 898 ATP.
Modified residue: 867 867 Phosphothreonine.

Protein Length

1173 AA.

Protein Sequence
(Canonical)

MKLLSKTFLI LTLTFFFFGI ALAKQSFEPE IEALKSFKNG ISNDPLGVLS DWTIIGSLRH  60
CNWTGITCDS TGHVVSVSLL EKQLEGVLSP AIANLTYLQV LDLTSNSFTG KIPAEIGKLT  120
ELNQLILYLN YFSGSIPSGI WELKNIFYLD LRNNLLSGDV PEEICKTSSL VLIGFDYNNL  180
TGKIPECLGD LVHLQMFVAA GNHLTGSIPV SIGTLANLTD LDLSGNQLTG KIPRDFGNLL  240
NLQSLVLTEN LLEGDIPAEI GNCSSLVQLE LYDNQLTGKI PAELGNLVQL QALRIYKNKL  300
TSSIPSSLFR LTQLTHLGLS ENHLVGPISE EIGFLESLEV LTLHSNNFTG EFPQSITNLR  360
NLTVLTVGFN NISGELPADL GLLTNLRNLS AHDNLLTGPI PSSISNCTGL KLLDLSHNQM  420
TGEIPRGFGR MNLTFISIGR NHFTGEIPDD IFNCSNLETL SVADNNLTGT LKPLIGKLQK  480
LRILQVSYNS LTGPIPREIG NLKDLNILYL HSNGFTGRIP REMSNLTLLQ GLRMYSNDLE  540
GPIPEEMFDM KLLSVLDLSN NKFSGQIPAL FSKLESLTYL SLQGNKFNGS IPASLKSLSL  600
LNTFDISDNL LTGTIPGELL ASLKNMQLYL NFSNNLLTGT IPKELGKLEM VQEIDLSNNL  660
FSGSIPRSLQ ACKNVFTLDF SQNNLSGHIP DEVFQGMDMI ISLNLSRNSF SGEIPQSFGN  720
MTHLVSLDLS SNNLTGEIPE SLANLSTLKH LKLASNNLKG HVPESGVFKN INASDLMGNT  780
DLCGSKKPLK PCTIKQKSSH FSKRTRVILI ILGSAAALLL VLLLVLILTC CKKKEKKIEN  840
SSESSLPDLD SALKLKRFEP KELEQATDSF NSANIIGSSS LSTVYKGQLE DGTVIAVKVL  900
NLKEFSAESD KWFYTEAKTL SQLKHRNLVK ILGFAWESGK TKALVLPFME NGNLEDTIHG  960
SAAPIGSLLE KIDLCVHIAS GIDYLHSGYG FPIVHCDLKP ANILLDSDRV AHVSDFGTAR  1020
ILGFREDGST TASTSAFEGT IGYLAPEFAY MRKVTTKADV FSFGIIMMEL MTKQRPTSLN  1080
DEDSQDMTLR QLVEKSIGNG RKGMVRVLDM ELGDSIVSLK QEEAIEDFLK LCLFCTSSRP  1140
EDRPDMNEIL THLMKLRGKA NSFREDRNED REV                               1173

FASTA
(Canonical)

>LipidDB-3702-01275|Q9FL28
MKLLSKTFLILTLTFFFFGIALAKQSFEPEIEALKSFKNGISNDPLGVLSDWTIIGSLRH
CNWTGITCDSTGHVVSVSLLEKQLEGVLSPAIANLTYLQVLDLTSNSFTGKIPAEIGKLT
ELNQLILYLNYFSGSIPSGIWELKNIFYLDLRNNLLSGDVPEEICKTSSLVLIGFDYNNL
TGKIPECLGDLVHLQMFVAAGNHLTGSIPVSIGTLANLTDLDLSGNQLTGKIPRDFGNLL
NLQSLVLTENLLEGDIPAEIGNCSSLVQLELYDNQLTGKIPAELGNLVQLQALRIYKNKL
TSSIPSSLFRLTQLTHLGLSENHLVGPISEEIGFLESLEVLTLHSNNFTGEFPQSITNLR
NLTVLTVGFNNISGELPADLGLLTNLRNLSAHDNLLTGPIPSSISNCTGLKLLDLSHNQM
TGEIPRGFGRMNLTFISIGRNHFTGEIPDDIFNCSNLETLSVADNNLTGTLKPLIGKLQK
LRILQVSYNSLTGPIPREIGNLKDLNILYLHSNGFTGRIPREMSNLTLLQGLRMYSNDLE
GPIPEEMFDMKLLSVLDLSNNKFSGQIPALFSKLESLTYLSLQGNKFNGSIPASLKSLSL
LNTFDISDNLLTGTIPGELLASLKNMQLYLNFSNNLLTGTIPKELGKLEMVQEIDLSNNL
FSGSIPRSLQACKNVFTLDFSQNNLSGHIPDEVFQGMDMIISLNLSRNSFSGEIPQSFGN
MTHLVSLDLSSNNLTGEIPESLANLSTLKHLKLASNNLKGHVPESGVFKNINASDLMGNT
DLCGSKKPLKPCTIKQKSSHFSKRTRVILIILGSAAALLLVLLLVLILTCCKKKEKKIEN
SSESSLPDLDSALKLKRFEPKELEQATDSFNSANIIGSSSLSTVYKGQLEDGTVIAVKVL
NLKEFSAESDKWFYTEAKTLSQLKHRNLVKILGFAWESGKTKALVLPFMENGNLEDTIHG
SAAPIGSLLEKIDLCVHIASGIDYLHSGYGFPIVHCDLKPANILLDSDRVAHVSDFGTAR
ILGFREDGSTTASTSAFEGTIGYLAPEFAYMRKVTTKADVFSFGIIMMELMTKQRPTSLN
DEDSQDMTLRQLVEKSIGNGRKGMVRVLDMELGDSIVSLKQEEAIEDFLKLCLFCTSSRP
EDRPDMNEILTHLMKLRGKANSFREDRNEDREV

Gene Ontology

GO:0005768; C:endosome; IDA:TAIR
GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW
GO:0016020; C:membrane; IDA:TAIR
GO:0005886; C:plasma membrane; IDA:TAIR
GO:0005524; F:ATP binding; IEA:UniProtKB-KW
GO:0004675; F:transmembrane receptor protein serine/threonine kinase activity; ISS:TAIR
GO:0052544; P:defense response by callose deposition in cell wall; IMP:TAIR
GO:0042742; P:defense response to bacterium; IMP:TAIR
GO:0016045; P:detection of bacterium; IMP:TAIR
GO:0006898; P:receptor-mediated endocytosis; IDA:TAIR
GO:0010359; P:regulation of anion channel activity; IMP:TAIR
GO:0007178; P:transmembrane receptor protein serine/threonine kinase signaling pathway; ISS:GOC

Interpro

InterPro; IPR013320; ConA-like_dom
InterPro; IPR011009; Kinase-like_dom
InterPro; IPR001611; Leu-rich_rpt
InterPro; IPR013210; LRR-contain_N2
InterPro; IPR000719; Prot_kinase_dom
InterPro; IPR008271; Ser/Thr_kinase_AS

Pfam

Pfam; PF00560; LRR_1;
Pfam; PF13855; LRR_8;
Pfam; PF08263; LRRNT_2;
Pfam; PF00069; Pkinase;

SMART

PROSITE

PROSITE; PS50011; PROTEIN_KINASE_DOM;
PROSITE; PS00108; PROTEIN_KINASE_ST;

PRINTS