LipidDB ID

LipidDB-3702-00406

Entry Name

UniProt Accession

P18064;

Theoretical PI

5.96

Molecular Weight

44545.91

Genbank Protein ID

AAA32805.1; AAC14520.1; AEC07820.1; AAM16227.1; AAK60296.1;

Genbank Nucleotide ID

M32887; AC004484; CP002685; AY093966; AF385704;

Protein Name

Guanine nucleotide-binding protein alpha-1 subunit

Protein Synonyms/Alias

GP-alpha-1;

Gene Name

GPA1

Gene Synonyms/Alias

At2g26300; T1D16.6;

Created Date

01-NOV-1990

Organism

Arabidopsis thaliana (Mouse-ear cress)

NCBI Taxa ID

3702

Reference

[1] Predicted from GPS-Lipid
[2] Boisson B, Giglione C, Meinnel T. Unexpected protein families including celldefense components feature in the N-myristoylome of a higher eukaryote. J BiolChem. 2003 Oct 31;278(44):43418-29. Epub 2003 Aug 11.[PMID:12912986]

Functional Description

Exhibits a fast rate of basal nucleotide exchange. Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Together with GCR1, may regulate the cell cycle via a signaling cascade that uses phosphatidylinositol-specific phospholipase C (PI-PLC) as an effector and inositol 1,4,5- trisphosphate(IP(3)) as a second messenger. Promotes abscisic acid (ABA) responses in guard cells. But, together with GCR1 and GB1, acts as a negative regulator of ABA during seed germination and early seedling development. Involved in the blue light (BL) signaling. Together with GCR1 and ADT3, required for BL-mediated synthesis of phenylpyruvate and subsequently of phenylalanine (Phe), in etiolated seedlings. Modulates root architecture (e.g. lateral root formation). Negatively regulated by RGS1.

Sequence Annotation

Nucleotide-binding: 45 52 GTP.
Nucleotide-binding: 187 193 GTP.
Nucleotide-binding: 218 222 GTP.
Nucleotide-binding: 287 290 GTP.
Metal binding site: 52 52 Magnesium.
Metal binding site: 193 193 Magnesium.
Binding site: 355 355 GTP; via amide nitrogen.

Protein Length

383 AA.

Protein Sequence
(Canonical)

FASTA
(Canonical)

Gene Ontology

GO:0005789; C:endoplasmic reticulum membrane; IDA:TAIR
GO:0031234; C:extrinsic component of cytoplasmic side of plasma membrane; IBA:RefGenome
GO:0005834; C:heterotrimeric G-protein complex; IDA:UniProtKB
GO:0005886; C:plasma membrane; IDA:UniProtKB
GO:0009506; C:plasmodesma; IDA:TAIR
GO:0016247; F:channel regulator activity; IMP:TAIR
GO:0031683; F:G-protein beta/gamma-subunit complex binding; IBA:RefGenome
GO:0001664; F:G-protein coupled receptor binding; IBA:RefGenome
GO:0005525; F:GTP binding; IDA:UniProtKB
GO:0003924; F:GTPase activity; IDA:TAIR
GO:0051020; F:GTPase binding; IPI:TAIR
GO:0005095; F:GTPase inhibitor activity; IDA:TAIR
GO:0046872; F:metal ion binding; IEA:UniProtKB-KW
GO:0004871; F:signal transducer activity; IBA:RefGenome
GO:0009738; P:abscisic acid-activated signaling pathway; IMP:UniProtKB
GO:0007188; P:adenylate cyclase-modulating G-protein coupled receptor signaling pathway; IBA:RefGenome
GO:0009785; P:blue light signaling pathway; IMP:UniProtKB
GO:0008219; P:cell death; IMP:TAIR
GO:0071215; P:cellular response to abscisic acid stimulus; IEP:UniProtKB
GO:0001789; P:G-protein coupled receptor signaling pathway, coupled to S1P second messenger; IMP:TAIR
GO:0009740; P:gibberellic acid mediated signaling pathway; IMP:TAIR
GO:0009094; P:L-phenylalanine biosynthetic process; IMP:TAIR
GO:0009788; P:negative regulation of abscisic acid-activated signaling pathway; IMP:UniProtKB
GO:0043086; P:negative regulation of catalytic activity; IDA:GOC
GO:0009789; P:positive regulation of abscisic acid-activated signaling pathway; IMP:TAIR
GO:0072593; P:reactive oxygen species metabolic process; IMP:TAIR
GO:0042127; P:regulation of cell proliferation; IMP:TAIR
GO:0010119; P:regulation of stomatal movement; IMP:TAIR
GO:0009749; P:response to glucose; IGI:TAIR
GO:0010244; P:response to low fluence blue light stimulus by blue low-fluence system; IMP:TAIR
GO:0009845; P:seed germination; IEP:UniProtKB
GO:0010027; P:thylakoid membrane organization; IMP:TAIR
GO:0006571; P:tyrosine biosynthetic process; IMP:TAIR

Interpro

InterPro; IPR001019; Gprotein_alpha_su
InterPro; IPR011025; GproteinA_insert
InterPro; IPR027417; P-loop_NTPase
InterPro; IPR002976; Plant_Gprotein_alpha

Pfam

Pfam; PF00503; G-alpha;

SMART

SMART; SM00275; G_alpha;

PROSITE

PRINTS

PRINTS; PR00318; GPROTEINA;
PRINTS; PR01242; GPROTEINAPLT;