LipidDB ID

LipidDB-211044-00943

Entry Name

UniProt Accession

P06821; A4GXH7; Q20N37; Q5UAV4; Q5UAV5; Q5UAV6; Q84105; Q8JUU3;

Theoretical PI

5.64

Molecular Weight

11044.7

Genbank Protein ID

CAA24283.1; AAM75162.1; ABO21713.1; AAV41244.1; AAV41245.1; AAV41246.1; ABD77677.1;

Genbank Nucleotide ID

V01099; AF389121; EF467824; AY768951; AY768952; AY768953; CY009445;

Protein Name

Matrix protein 2

Protein Synonyms/Alias

Proton channel protein M2;

Gene Name

M

Gene Synonyms/Alias

Created Date

01-JAN-1988

Organism

Influenza A virus (strain A/Puerto Rico/8/1934 H1N1)

NCBI Taxa ID

211044

Reference

[1] Thaa B, Levental I, Herrmann A, Veit M. Intrinsic membrane association of the cytoplasmic tail of influenza virus M2 protein and lateral membrane sortingregulated by cholesterol binding and palmitoylation. Biochem J. 2011 Aug1;437(3):389-97. doi: 10.1042/BJ20110706.[PMID:21592088]

Functional Description

Forms a proton-selective ion channel that is necessary for the efficient release of the viral genome during virus entry. After attaching to the cell surface, the virion enters the cell by endocytosis. Acidification of the endosome triggers M2 ion channel activity. The influx of protons into virion interior is believed to disrupt interactions between the viral ribonucleoprotein (RNP), matrix protein 1 (M1), and lipid bilayers, thereby freeing the viral genome from interaction with viral proteins and enabling RNA segments to migrate to the host cell nucleus, where influenza virus RNA transcription and replication occur. Also plays a role in viral proteins secretory pathway. Elevates the intravesicular pH of normally acidic compartments, such as trans-Golgi network, preventing newly formed hemagglutinin from premature switching to the fusion-active conformation (By similarity).

Sequence Annotation

Topological domain: 1 22 Virion surface.
Transmembrane: 23 43 Helical; Signal-anchor for type IIImembrane protein.
Topological domain: 44 97 Intravirion.
Functional site: 37 37 Essential for channel activity, possiblyby being protonated during channelactivation, and by forming the channelgate and the selective filter.
Functional site: 41 41 Seems to be involved in pH gating.
Modified residue: 64 64 Phosphoserine; by host.
Modified residue: 82 82 Phosphoserine; by host.
Modified residue: 93 93 Phosphoserine; by host.

Protein Length

97 AA.

Protein Sequence
(Canonical)

FASTA
(Canonical)

Gene Ontology

GO:0005829; C:cytosol; TAS:Reactome
GO:0030666; C:endocytic vesicle membrane; TAS:Reactome
GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome
GO:0031904; C:endosome lumen; TAS:Reactome
GO:0005576; C:extracellular region; TAS:Reactome
GO:0000139; C:Golgi membrane; TAS:Reactome
GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-KW
GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW
GO:0044385; C:integral to membrane of host cell; IEA:UniProtKB-KW
GO:0005886; C:plasma membrane; TAS:Reactome
GO:0055036; C:virion membrane; IEA:InterPro
GO:0015078; F:hydrogen ion transmembrane transporter activity; IEA:InterPro
GO:0005216; F:ion channel activity; IEA:UniProtKB-KW
GO:0005215; F:transporter activity; TAS:Reactome
GO:0019064; P:fusion of virus membrane with host plasma membrane; TAS:Reactome
GO:0019060; P:intracellular transport of viral protein in host cell; TAS:Reactome
GO:0039707; P:pore formation by virus in membrane of host cell; IEA:UniProtKB-KW
GO:0051259; P:protein oligomerization; IEA:UniProtKB-KW
GO:0019065; P:receptor-mediated endocytosis of virus by host cell; TAS:Reactome
GO:0039521; P:suppression by virus of host autophagy; IEA:UniProtKB-KW
GO:0019061; P:uncoating of virus; TAS:Reactome
GO:0046718; P:viral entry into host cell; TAS:Reactome
GO:0019072; P:viral genome packaging; TAS:Reactome
GO:0019058; P:viral life cycle; TAS:Reactome
GO:0016032; P:viral process; TAS:Reactome
GO:0019076; P:viral release from host cell; TAS:Reactome
GO:0019083; P:viral transcription; TAS:Reactome
GO:0019068; P:virion assembly; TAS:Reactome

Interpro

InterPro; IPR002089; Flu_M2

Pfam

Pfam; PF00599; Flu_M2;

SMART

PROSITE

PRINTS