LipidDB ID

LipidDB-11707-00206

Entry Name

UniProt Accession

P05854; Q85588;

Theoretical PI

6.31

Molecular Weight

23419.43

Genbank Protein ID

CAA26947.1; AAA44680.1;

Genbank Nucleotide ID

X03188; M14100;

Protein Name

C-terminal core protein

Protein Synonyms/Alias

3'ORF; Negative factor; F-protein;

Gene Name

nef

Gene Synonyms/Alias

Created Date

01-NOV-1988

Organism

Human immunodeficiency virus type 1 group M subtype B (isolate HXB3 (HIV-1)

NCBI Taxa ID

11707

Reference

[1] Yu G, Felsted RL. Effect of myristoylation on p27 nef subcellular distributionand suppression of HIV-LTR transcription. Virology. 1992 Mar;187(1):46-55. PubMedPMID: 1736544.[PMID:1736544]
[2] Geyer M, Munte CE, Schorr J, Kellner R, Kalbitzer HR. Structure of theanchor-domain of myristoylated and non-myristoylated HIV-1 Nef protein. J MolBiol. 1999 May 28;289(1):123-38.[PMID:10339411]

Functional Description

Factor of infectivity and pathogenicity, required for optimal virus replication. Alters numerous pathways of T- lymphocytes function and down-regulates immunity surface molecules in order to evade host defense and increase viral infectivity. Alters the functionality of other immunity cells, like dendritic cells, monocytes/macrophages and NK cells. One of the earliest and most abundantly expressed viral proteins (By similarity).

Sequence Annotation

Region: 2 57 N-terminal; associates with the hostplasma membrane.
Region: 7 26 Necessary for MHC-I internalization.
Region: 62 65 Acidic; stabilizes the interaction ofNEF/MHC-I with host AP1M1; necessary forMHC-I internalization and interactionwith host PACS1 and PACS2.
Region: 69 78 SH3-binding; interaction with Src familytyrosine kinases.
Region: 108 124 Mediates dimerization, Nef-PTE1interaction, Nef-induced CD4 and MHC-Idown-regulation and enhancement ofinfectivity.
Region: 148 180 Binding to ATP6V1H.
Motif: 72 75 PxxP; stabilizes the interaction ofNEF/MHC-I with host AP1M1; necessary forMHC-I internalization.
Motif: 164 165 Di-leucine internalization motif;necessary for CD4 internalization.
Motif: 174 175 Diacidic; necessary for CD4internalization.
Functional site: 20 20 Might play a role in AP-1 recruitment tothe Nef-MHC-I complex.
Functional site: 57 58 Cleavage; by viral protease.

Protein Length

206 AA.

Protein Sequence
(Canonical)

FASTA
(Canonical)

Gene Ontology

GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-KW
GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-KW
GO:0016020; C:membrane; IEA:UniProtKB-KW
GO:0019012; C:virion; IEA:UniProtKB-KW
GO:0005525; F:GTP binding; IEA:InterPro
GO:0006915; P:apoptotic process; IEA:UniProtKB-KW
GO:0009405; P:pathogenesis; IEA:UniProtKB-KW
GO:0039504; P:suppression by virus of host adaptive immune response; IEA:UniProtKB-KW
GO:0046776; P:suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I; IEA:UniProtKB-KW
GO:0039505; P:suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II; IEA:UniProtKB-KW
GO:0039521; P:suppression by virus of host autophagy; IEA:UniProtKB-KW

Interpro

InterPro; IPR027480; HIV-1_Nef_anchor
InterPro; IPR027481; HIV-1_Nef_core
InterPro; IPR001558; HIV_Nef

Pfam

Pfam; PF00469; F-protein;

SMART

PROSITE

PRINTS