LipidDB ID

LipidDB-10633-01359

Entry Name

UniProt Accession

P03093;

Theoretical PI

6.99

Molecular Weight

38539.36

Genbank Protein ID

AAB59921.1; AAB59922.1;

Genbank Nucleotide ID

J02400; J02400;

Protein Name

Minor capsid protein VP2

Protein Synonyms/Alias

Minor structural protein VP2;

Gene Name

Gene Synonyms/Alias

Created Date

21-JUL-1986

Organism

Simian virus 40 (SV40)

NCBI Taxa ID

10633

Reference

[1] Streuli CH, Griffin BE. Myristic acid is coupled to a structural protein ofpolyoma virus and SV40. Nature. 1987 Apr 9-15;326(6113):619-22.[PMID:3031509]

Functional Description

Isoform VP2 is a structural protein that resides within the core of the capsid surrounded by 72 VP1 pentamers. Following virus endocytosis and trafficking to the endoplasmic reticulum, VP2 and VP3 form oligomers and integrate into the endoplasmic reticulum membrane. Heterooligomer VP2-VP3 may create a viroporin for transporting the viral genome across the endoplasmic reticulum membrane to the cytoplasm. Nuclear entry of the viral DNA involves the selective exposure and importin recognition of VP2 or Vp3 nuclear localization signal (shared C-terminus). Plays a role in virion assembly within the nucleus in particular through a DNA- binding domain located in the C-terminal region. A N-terminal myristoylation suggests a scaffold function for virion assembly. The viral progenies exit the cells by lytic release. Isoform VP2 may repress SP1 activation of the SV40 early promoter, via specific protein-protein and protein-DNA interactions.Isoform VP4 is a viroporin inducing perforation of cellular membranes to trigger virus progeny release. Forms pores of 3 nm inner diameter. VP4 is expressed about 24 hours after the late structural proteins and is not incorporated into the mature virion.

Sequence Annotation

Transmembrane: 290 310 Helical.
Region: 273 308 D1.
Region: 313 352 DNA-binding.
Motif: 316 324 Nuclear localization signal.

Protein Length

352 AA.

Protein Sequence
(Canonical)

FASTA
(Canonical)

Gene Ontology

GO:0044165; C:host cell endoplasmic reticulum; IEA:UniProtKB-KW
GO:0042025; C:host cell nucleus; IEA:UniProtKB-KW
GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW
GO:0044385; C:integral to membrane of host cell; IEA:UniProtKB-KW
GO:0005622; C:intracellular; IEA:GOC
GO:0019028; C:viral capsid; IEA:UniProtKB-KW
GO:0003677; F:DNA binding; IEA:UniProtKB-KW
GO:0005216; F:ion channel activity; IEA:UniProtKB-KW
GO:0005198; F:structural molecule activity; IEA:InterPro
GO:0039665; P:permeabilization of host organelle membrane involved in viral entry into host cell; IEA:UniProtKB-KW
GO:0039707; P:pore formation by virus in membrane of host cell; IEA:UniProtKB-KW
GO:0051259; P:protein oligomerization; IEA:UniProtKB-KW
GO:0075732; P:viral penetration into host nucleus; IEA:UniProtKB-KW
GO:0019076; P:viral release from host cell; IEA:UniProtKB-KW
GO:0019062; P:virion attachment to host cell; IEA:UniProtKB-KW

Interpro

InterPro; IPR001070; Polyoma_coat_VP2

Pfam

Pfam; PF00761; Polyoma_coat2;

SMART

PROSITE

PRINTS