LipidDB ID

LipidDB-10116-00818

Entry Name

UniProt Accession

P49187;

Theoretical PI

6.37

Molecular Weight

52530.35

Genbank Protein ID

AAA42110.1;

Genbank Nucleotide ID

L27128;

Protein Name

Mitogen-activated protein kinase 10

Protein Synonyms/Alias

MAP kinase 10; MAPK 10; 2.7.11.24; SAPK-beta; Stress-activated protein kinase JNK3; c-Jun N-terminal kinase 3; p54-beta;

Gene Name

Mapk10

Gene Synonyms/Alias

Jnk3; Prkm10;

Created Date

01-FEB-1996

Organism

Rattus norvegicus (Rat)

NCBI Taxa ID

10116

Reference

[1] Yang G, Liu Y, Yang K, Liu R, Zhu S, Coquinco A, Wen W, Kojic L, Jia W,Cynader M. Isoform-specific palmitoylation of JNK regulates axonal development.Cell Death Differ. 2012 Apr;19(4):553-61. doi: 10.1038/cdd.2011.124. Epub 2011Sep 23.[PMID:21941371]
[2] Yang G, Zhou X, Zhu J, Liu R, Zhang S, Coquinco A, Chen Y, Wen Y, Kojic L, JiaW, Cynader MS. JNK3 couples the neuronal stress response to inhibition ofsecretory trafficking. Sci Signal. 2013 Jul 9;6(283):ra57. doi:10.1126/scisignal.2003727.[PMID:23838184]

Functional Description

Serine/threonine-protein kinase involved in various processes such as neuronal proliferation, differentiation, migration and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress- activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK10/JNK3. In turn, MAPK10/JNK3 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. Plays regulatory roles in the signaling pathways during neuronal apoptosis. Phosphorylates the neuronal microtubule regulator STMN2. Acts in the regulation of the beta-amyloid precursor protein/APP signaling during neuronal differentiation by phosphorylating APP. Participates also in neurite growth in spiral ganglion neurons (By similarity).

Sequence Annotation

Domain: 64 359 Protein kinase.
Nucleotide-binding: 70 78 ATP.
Motif: 221 223 TXY.
Active site: 189 189 Proton acceptor.
Binding site: 93 93 ATP.
Modified residue: 154 154 S-nitrosocysteine.
Modified residue: 221 221 Phosphothreonine; by MAP2K7.
Modified residue: 223 223 Phosphotyrosine; by MAP2K4.

Protein Length

464 AA.

Protein Sequence
(Canonical)

FASTA
(Canonical)

Gene Ontology

GO:0005737; C:cytoplasm; ISS:UniProtKB
GO:0005739; C:mitochondrion; ISS:UniProtKB
GO:0005634; C:nucleus; IEA:UniProtKB-KW
GO:0005886; C:plasma membrane; ISS:UniProtKB
GO:0005524; F:ATP binding; IDA:RGD
GO:0004705; F:JUN kinase activity; ISS:UniProtKB
GO:0007254; P:JNK cascade; ISS:UniProtKB
GO:0007258; P:JUN phosphorylation; IDA:RGD
GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:ParkinsonsUK-UCL
GO:0006468; P:protein phosphorylation; IDA:RGD

Interpro

InterPro; IPR011009; Kinase-like_dom
InterPro; IPR003527; MAP_kinase_CS
InterPro; IPR008351; MAPK_JNK
InterPro; IPR000719; Prot_kinase_dom
InterPro; IPR002290; Ser/Thr_dual-sp_kinase
InterPro; IPR008271; Ser/Thr_kinase_AS

Pfam

Pfam; PF00069; Pkinase;

SMART

SMART; SM00220; S_TKc;

PROSITE

PROSITE; PS01351; MAPK;
PROSITE; PS50011; PROTEIN_KINASE_DOM;
PROSITE; PS00108; PROTEIN_KINASE_ST;

PRINTS

PRINTS; PR01772; JNKMAPKINASE;