LipidDB ID

LipidDB-10116-00150

Entry Name

UniProt Accession

P21707; Q3S2E6; Q707P5;

Theoretical PI

8.57

Molecular Weight

47398.95

Genbank Protein ID

CAA36981.1; CAE85101.1; ABA00482.1;

Genbank Nucleotide ID

X52772; AJ617615; DQ181550;

Protein Name

Synaptotagmin-1

Protein Synonyms/Alias

Synaptotagmin I; SytI; p65;

Gene Name

Syt1

Gene Synonyms/Alias

Created Date

01-MAY-1991

Organism

Rattus norvegicus (Rat)

NCBI Taxa ID

10116

Reference

[1] Heindel U, Schmidt MF, Veit M. Palmitoylation sites and processing ofsynaptotagmin I, the putative calcium sensor for neurosecretion. FEBS Lett. 2003 Jun 5;544(1-3):57-62.[PMID:12782290]

Functional Description

May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.

Sequence Annotation

Topological domain: 1 57 Vesicular.
Transmembrane: 58 79 Helical.
Topological domain: 80 421 Cytoplasmic.
Domain: 143 244 C2 1.
Domain: 274 377 C2 2.
Region: 135 381 Phospholipid binding.
Metal binding site: 171 171 Calcium 2; via carbonyl oxygen.
Metal binding site: 172 172 Calcium 1.
Metal binding site: 172 172 Calcium 2.
Metal binding site: 178 178 Calcium 1.
Metal binding site: 230 230 Calcium 1.
Metal binding site: 230 230 Calcium 2.
Metal binding site: 231 231 Calcium 1; via carbonyl oxygen.
Metal binding site: 232 232 Calcium 1.
Metal binding site: 232 232 Calcium 2.
Metal binding site: 232 232 Calcium 3.
Metal binding site: 235 235 Calcium 3.
Metal binding site: 236 236 Calcium 3; via carbonyl oxygen.
Metal binding site: 238 238 Calcium 2.
Metal binding site: 238 238 Calcium 3.
Modified residue: 128 128 Phosphothreonine.
Modified residue: 229 229 Phosphotyrosine.

Protein Length

421 AA.

Protein Sequence
(Canonical)

FASTA
(Canonical)

Gene Ontology

GO:0030054; C:cell junction; IEA:UniProtKB-KW
GO:0005737; C:cytoplasm; IDA:MGI
GO:0031045; C:dense core granule; IDA:MGI
GO:0060076; C:excitatory synapse; IDA:BHF-UCL
GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW
GO:0043229; C:intracellular organelle; IDA:RGD
GO:0043005; C:neuron projection; IEA:Ensembl
GO:0005886; C:plasma membrane; IDA:BHF-UCL
GO:0042734; C:presynaptic membrane; IEA:Ensembl
GO:0030141; C:secretory granule; IDA:RGD
GO:0008021; C:synaptic vesicle; IDA:BHF-UCL
GO:0030672; C:synaptic vesicle membrane; TAS:RGD
GO:0005509; F:calcium ion binding; IDA:RGD
GO:0005544; F:calcium-dependent phospholipid binding; IDA:BHF-UCL
GO:0048306; F:calcium-dependent protein binding; IDA:RGD
GO:0005516; F:calmodulin binding; IDA:RGD
GO:0030276; F:clathrin binding; IDA:BHF-UCL
GO:0042802; F:identical protein binding; IPI:IntAct
GO:0005546; F:phosphatidylinositol-4,5-bisphosphate binding; IDA:RGD
GO:0001786; F:phosphatidylserine binding; IDA:RGD
GO:0005543; F:phospholipid binding; IDA:RGD
GO:0008022; F:protein C-terminus binding; IPI:RGD
GO:0000149; F:SNARE binding; IPI:ParkinsonsUK-UCL
GO:0019905; F:syntaxin binding; IDA:BHF-UCL
GO:0017075; F:syntaxin-1 binding; IDA:ParkinsonsUK-UCL
GO:0005215; F:transporter activity; IEA:InterPro
GO:0017156; P:calcium ion-dependent exocytosis; TAS:RGD
GO:0048791; P:calcium ion-dependent exocytosis of neurotransmitter; ISS:ParkinsonsUK-UCL
GO:0005513; P:detection of calcium ion; IDA:RGD
GO:0045956; P:positive regulation of calcium ion-dependent exocytosis; IDA:RGD
GO:0050806; P:positive regulation of synaptic transmission; ISS:ParkinsonsUK-UCL
GO:0031340; P:positive regulation of vesicle fusion; IDA:RGD
GO:0017158; P:regulation of calcium ion-dependent exocytosis; IMP:RGD
GO:1903305; P:regulation of regulated secretory pathway; IMP:ParkinsonsUK-UCL
GO:0051966; P:regulation of synaptic transmission, glutamatergic; ISS:ParkinsonsUK-UCL
GO:0051592; P:response to calcium ion; IDA:RGD
GO:0016079; P:synaptic vesicle exocytosis; TAS:RGD
GO:0048278; P:vesicle docking; IDA:RGD

Interpro

InterPro; IPR000008; C2_dom
InterPro; IPR001565; Synaptotagmin
InterPro; IPR015428; Synaptotagmin1

Pfam

Pfam; PF00168; C2;

SMART

SMART; SM00239; C2;

PROSITE

PROSITE; PS50004; C2;

PRINTS

PRINTS; PR00360; C2DOMAIN;
PRINTS; PR00399; SYNAPTOTAGMN;