LipidDB ID

LipidDB-10090-00758

Entry Name

UniProt Accession

O35598; B8JJJ0;

Theoretical PI

8.27

Molecular Weight

83968.11

Genbank Protein ID

AAC53303.1; CAX15709.1; CAX15709.1; CAX15709.1; EDL26211.1; BAC29620.1; BAC29502.1;

Genbank Nucleotide ID

AF011379; CT025701; AC091263; AC160636; CH466522; AK036883; AK036599;

Protein Name

Disintegrin and metalloproteinase domain-containing protein 10

Protein Synonyms/Alias

ADAM 10; 3.4.24.81; Kuzbanian protein homolog; Mammalian disintegrin-metalloprotease; CD156c;

Gene Name

Adam10

Gene Synonyms/Alias

Kuz; Madm;

Created Date

28-FEB-2003

Organism

Mus musculus (Mouse)

NCBI Taxa ID

10090

Reference

[1] Predicted from GPS-Lipid

Functional Description

Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface (By similarity). Responsible for the proteolytic release of several other cell- surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha- secretase cleavage of amyloid precursor protein (APP) at '687- Lys-|-Leu-688'. Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10- processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling (By similarity).

Sequence Annotation

Topological domain: 20 673 Extracellular.
Transmembrane: 674 694 Helical.
Topological domain: 695 749 Cytoplasmic.
Domain: 221 457 Peptidase M12B.
Domain: 458 552 Disintegrin.
Motif: 171 178 Cysteine switch.
Motif: 709 716 SH3-binding.
Motif: 723 729 SH3-binding.
Active site: 385 385
Metal binding site: 173 173 Zinc; in inhibited form.
Metal binding site: 384 384 Zinc; catalytic.
Metal binding site: 388 388 Zinc; catalytic.
Metal binding site: 394 394 Zinc; catalytic.

Protein Length

749 AA.

Protein Sequence
(Canonical)

FASTA
(Canonical)

Gene Ontology

GO:0009986; C:cell surface; ISS:UniProtKB
GO:0005737; C:cytoplasm; IDA:UniProtKB
GO:0070062; C:extracellular vesicular exosome; IEA:Ensembl
GO:0005794; C:Golgi apparatus; ISS:UniProtKB
GO:0005798; C:Golgi-associated vesicle; ISS:UniProtKB
GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW
GO:0005634; C:nucleus; IDA:UniProtKB
GO:0097038; C:perinuclear endoplasmic reticulum; IEA:Ensembl
GO:0005886; C:plasma membrane; TAS:Reactome
GO:0014069; C:postsynaptic density; IDA:MGI
GO:0097197; C:tetraspanin-enriched microdomain; IEA:Ensembl
GO:0004175; F:endopeptidase activity; IDA:MGI
GO:0004222; F:metalloendopeptidase activity; ISS:UniProtKB
GO:0008237; F:metallopeptidase activity; IMP:UniProtKB
GO:0042803; F:protein homodimerization activity; IPI:UniProtKB
GO:0019901; F:protein kinase binding; IDA:UniProtKB
GO:0042169; F:SH2 domain binding; NAS:UniProtKB
GO:0008270; F:zinc ion binding; IEA:InterPro
GO:0051089; P:constitutive protein ectodomain proteolysis; IEA:Ensembl
GO:0001701; P:in utero embryonic development; IMP:UniProtKB
GO:0006509; P:membrane protein ectodomain proteolysis; ISS:UniProtKB
GO:0042117; P:monocyte activation; IEA:Ensembl
GO:0007162; P:negative regulation of cell adhesion; ISS:UniProtKB
GO:0007220; P:Notch receptor processing; NAS:UniProtKB
GO:0007219; P:Notch signaling pathway; IMP:UniProtKB
GO:0006913; P:nucleocytoplasmic transport; NAS:UniProtKB
GO:0051088; P:PMA-inducible membrane protein ectodomain proteolysis; IEA:Ensembl
GO:0030307; P:positive regulation of cell growth; IEA:Ensembl
GO:0008284; P:positive regulation of cell proliferation; IEA:Ensembl
GO:0010820; P:positive regulation of T cell chemotaxis; IEA:Ensembl
GO:0006468; P:protein phosphorylation; IDA:UniProtKB
GO:0034612; P:response to tumor necrosis factor; IEA:Ensembl

Interpro

InterPro; IPR027053; ADAM_10
InterPro; IPR001762; Blood-coag_inhib_Disintegrin
InterPro; IPR024079; MetalloPept_cat_dom
InterPro; IPR001590; Peptidase_M12B
InterPro; IPR002870; Peptidase_M12B_N

Pfam

Pfam; PF00200; Disintegrin;
Pfam; PF01562; Pep_M12B_propep;

SMART

SMART; SM00050; DISIN;

PROSITE

PROSITE; PS50215; ADAM_MEPRO;
PROSITE; PS50214; DISINTEGRIN_2;
PROSITE; PS00142; ZINC_PROTEASE;

PRINTS