LipidDB-10090-00448

Lipid Modification Database

Tag

Content

LipidDB ID

LipidDB-10090-00448

Entry Name

GNAS2_MOUSE

UniProt Accession

P63094; A2A611; A2A612; A2A613; P04894; P08755; Q3KQM5; Q3TFV3; Q3TWS9; Q3UI70; Q58E62; Q6P7U9; Q80ZK6; Q8K5E1; Q9Z1R7;

Theoretical PI

5.69

Molecular Weight

45663.6

Genbank Protein ID

CAA68695.1; AAA37745.1; AAD11807.1; BAE27636.1; BAE35187.1; BAE40795.1; CAM24410.1; CAM24411.1; CAM24412.1; AAH38067.1; AAH48834.1; AAH61496.1; AAH62654.1; AAH80816.1; AAH92055.1; AAI06134.1; BAB93551.1;

Genbank Nucleotide ID

Y00703; M13964; AF107848; AK147051; AK159563; AK168996; AL593857; AL593857; AL593857; BC038067; BC048834; BC061496; BC062654; BC080816; BC092055; BC106133; AB041808;

Protein Name

Guanine nucleotide-binding protein G(s) subunit alpha isoforms short

Protein Synonyms/Alias

Adenylate cyclase-stimulating G alpha protein;

Gene Name

Gnas

Gene Synonyms/Alias

Gnas1; MNCb-5546;

Created Date

13-AUG-1987

Lipid Modification Sites
Position	Sequence Form	Peptide	References	Modification Type
3	Canonical	*****MGCLGNSKTE	[1]	S-Palmitoylation

Organism

Mus musculus (Mouse)

NCBI Taxa ID

10090

Reference

[1] Wedegaertner PB, Chu DH, Wilson PT, Levis MJ, Bourne HR. Palmitoylation isrequired for signaling functions and membrane attachment of Gq alpha and Gsalpha. J Biol Chem. 1993 Nov 25;268(33):25001-8.[PMID:8227063]

Functional Description

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(s) protein is involved in hormonal regulation of adenylate cyclase: it activates the cyclase in response to beta-adrenergic stimuli. Stimulates the Ras signaling pathway via RAPGEF2.

Sequence Annotation

Nucleotide-binding: 47 54 GTP.
Nucleotide-binding: 198 204 GTP.
Nucleotide-binding: 223 227 GTP.
Nucleotide-binding: 292 295 GTP.
Metal binding site: 54 54 Magnesium.
Metal binding site: 204 204 Magnesium.
Binding site: 366 366 GTP; via amide nitrogen.
Modified residue: 352 352 Phosphoserine.

Protein Length

394 AA.

Protein Sequence
(Canonical)

MGCLGNSKTE DQRNEEKAQR EANKKIEKQL QKDKQVYRAT HRLLLLGAGE SGKSTIVKQM  60
RILHVNGFNG EGGEEDPQAA RSNSDGEKAT KVQDIKNNLK EAIETIVAAM SNLVPPVELA  120
NPENQFRVDY ILSVMNVPNF DFPPEFYEHA KALWEDEGVR ACYERSNEYQ LIDCAQYFLD  180
KIDVIKQADY VPSDQDLLRC RVLTSGIFET KFQVDKVNFH MFDVGGQRDE RRKWIQCFND  240
VTAIIFVVAS SSYNMVIRED NQTNRLQEAL NLFKSIWNNR WLRTISVILF LNKQDLLAEK  300
VLAGKSKIED YFPEFARYTT PEDATPEPGE DPRVTRAKYF IRDEFLRIST ASGDGRHYCY  360
PHFTCAVDTE NIRRVFNDCR DIIQRMHLRQ YELL                              394

FASTA
(Canonical)

>LipidDB-10090-00448|P63094
MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQM
RILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELA
NPENQFRVDYILSVMNVPNFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLD
KIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFND
VTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEK
VLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCY
PHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Gene Ontology

GO:0030425; C:dendrite; IDA:MGI
GO:0005834; C:heterotrimeric G-protein complex; ISA:MGI
GO:0016020; C:membrane; IDA:MGI
GO:0031698; F:beta-2 adrenergic receptor binding; IBA:RefGenome
GO:0051430; F:corticotropin-releasing hormone receptor 1 binding; IBA:RefGenome
GO:0031748; F:D1 dopamine receptor binding; IBA:RefGenome
GO:0031683; F:G-protein beta/gamma-subunit complex binding; IBA:RefGenome
GO:0005525; F:GTP binding; IEA:UniProtKB-KW
GO:0003924; F:GTPase activity; ISA:MGI
GO:0005159; F:insulin-like growth factor receptor binding; IBA:RefGenome
GO:0035255; F:ionotropic glutamate receptor binding; IBA:RefGenome
GO:0046872; F:metal ion binding; IEA:UniProtKB-KW
GO:0031852; F:mu-type opioid receptor binding; IBA:RefGenome
GO:0004871; F:signal transducer activity; ISS:UniProtKB
GO:0071880; P:adenylate cyclase-activating adrenergic receptor signaling pathway; ISS:UniProtKB
GO:0007191; P:adenylate cyclase-activating dopamine receptor signaling pathway; IGI:MGI
GO:0007189; P:adenylate cyclase-activating G-protein coupled receptor signaling pathway; IDA:MGI
GO:0051216; P:cartilage development; IMP:MGI
GO:0006306; P:DNA methylation; IMP:MGI
GO:0048701; P:embryonic cranial skeleton morphogenesis; IMP:MGI
GO:0035116; P:embryonic hindlimb morphogenesis; IMP:MGI
GO:0001958; P:endochondral ossification; IMP:MGI
GO:0006112; P:energy reserve metabolic process; IMP:MGI
GO:0007186; P:G-protein coupled receptor signaling pathway; TAS:MGI
GO:0071514; P:genetic imprinting; IMP:MGI
GO:0035264; P:multicellular organism growth; IMP:MGI
GO:0030819; P:positive regulation of cAMP biosynthetic process; ISS:UniProtKB
GO:0043950; P:positive regulation of cAMP-mediated signaling; ISS:UniProtKB
GO:0045669; P:positive regulation of osteoblast differentiation; IMP:MGI
GO:0045672; P:positive regulation of osteoclast differentiation; IMP:MGI
GO:0032320; P:positive regulation of Ras GTPase activity; ISS:UniProtKB
GO:0040032; P:post-embryonic body morphogenesis; IMP:MGI
GO:0009791; P:post-embryonic development; IMP:MGI
GO:0042493; P:response to drug; IMP:MGI
GO:0007606; P:sensory perception of chemical stimulus; IBA:RefGenome
GO:0001501; P:skeletal system development; IMP:MGI
GO:0043588; P:skin development; IMP:MGI
GO:0001894; P:tissue homeostasis; IMP:MGI

Interpro

InterPro; IPR000367; Gprotein_alpha_S
InterPro; IPR001019; Gprotein_alpha_su
InterPro; IPR011025; GproteinA_insert
InterPro; IPR027417; P-loop_NTPase

Pfam

Pfam; PF00503; G-alpha;

SMART

SMART; SM00275; G_alpha;

PROSITE

PRINTS

PRINTS; PR00318; GPROTEINA;
PRINTS; PR00443; GPROTEINAS;